MIXED CARBON PASTE PEA SEEDLING ELECTROCHEMICAL SENSOR FOR MEASURING PLANT GROWTH-REGULATING ACTIVITY OF AMINES

被引：13

作者：

WIJESURIYA, D

RECHNITZ, GA

机构：

[1] Hawaii Biosensor Laboratory, Department of Chemistry, University of Hawaii at Manoa, Honolulu, HI 96822

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 243卷 / 01期

基金：

美国国家科学基金会;

关键词：

AMPEROMETRY; ENZYME ELECTRODES; AMINES; PLANT GROWTH REGULATORS;

D O I：

10.1016/S0003-2670(00)82533-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of pea seedlings as a source of diamine oxidase for the determination of enzymatic oxidation rates, which are important in evaluating the plant growth-regulating activity, of various amines is reported. A mixed carbon paste-plant tissue amperometric sensor was constructed and characterized using speramidine as a substrate (K(M) = 5 x 10(-6) M). Generation of hydrogen peroxide due to enzymatic oxidation of amine was monitored at 0.9 V vs. Ag/AgCl under steady-state conditions using this sensor. Easy construction, reliability of the data, reusability, shorter response times (of the order of 4 s) and a detection limit of 7.1 nmol 1(-1) are some of the advantages.

引用

页码：1 / 8

页数：8

共 16 条

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