HETEROGENEOUS INDUCTION OF 72-KDA HEAT-SHOCK PROTEIN (HSP72) IN CULTURED MOUSE OLIGODENDROCYTES AND ASTROCYTES

被引:34
作者
SATOH, J
YAMAMURA, T
KUNISHITA, T
TABIRA, T
机构
[1] Division of Demyelinating Diseases and Aging, National Institute of Neuroscience, NCNP, Tokyo
关键词
ASTROCYTE; DOUBLE IMMUNOSTAINING; HSP72; HEAT STRESS; OLIGODENDROCYTE; SUBCELLULAR LOCATION;
D O I
10.1016/0006-8993(92)90111-L
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The expression of 72-kDa heat shock protein (HSP72) in cultured mouse oligodendrocytes and astrocytes exposed to heat shock was investigated by double immunolabelling with anti-HSP72 monoclonal antibody (C92F3B-1) and antibodies against galactocerebroside (GalC) or glial fibrillary acidic protein (GFAP). After 3 h recovery from heat shock, an intermediate level of HSP72 immunolabelling was localized in the nucleolus and cytoplasm of astrocytes (< 25%) and to a lesser extent in oligodendrocytes (< 2%). After 8-48 h, HSP72 was expressed intensely in the cytoplasm and nuclear matrix of oligodendrocytes (20-40%), while weak/intermediate immunostaining was detectable in astrocytes (5-15%). The levels of HSP72 expressed in oligodendrocytes and astrocytes decreased around 72-120 h, but a few oligodendrocytes (4%) remained intensely immunolabelled. These results indicate that heat shock induces HSP72 in both oligodendrocytes and astrocytes. However, this response is heterogeneous.
引用
收藏
页码:37 / 43
页数:7
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