ENANTIOMER SEPARATION OF DNP-AMINO ACIDS BY CAPILLARY ELECTROPHORESIS USING CHIRAL BUFFER ADDITIVES

被引：34

作者：

WISTUBA, D ^{[1
]}

DIEBOLD, H ^{[1
]}

SCHURIG, V ^{[1
]}

机构：

[1] UNIV TUBINGEN,INST ORGAN CHEM,D-72076 TUBINGEN,GERMANY

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1995年 / 7卷 / 01期

关键词：

ENANTIOMER SEPARATION; CAPILLARY ELECTROPHORESIS; CHIRAL BUFFER ADDITIVES; PROTEINS; CYCLODEXTRINS;

D O I：

10.1002/mcs.1220070103

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The direct enantiomeric analysis of six DNP-amino acids by capillary electrophoresis is reported. This study describes a comparison of several chiral buffer additives. Both the natural proteins BSA, AGP, ovomucoid and casein, and the cyclic oligosaccharides alpha-, beta-, and gamma-cyclodextrin were used as chiral selectors. For the first time, casein from goat milk was successfully applied for chiral discrimination. The influence of the modifier n-propanol on chiral discrimination is demonstrated with BSA as a buffer additive.

引用

页码：17 / 22

页数：6

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