ENANTIOMER SEPARATION OF DNP-AMINO ACIDS BY CAPILLARY ELECTROPHORESIS USING CHIRAL BUFFER ADDITIVES

被引：34

作者：

WISTUBA, D ^{[1
]}

DIEBOLD, H ^{[1
]}

SCHURIG, V ^{[1
]}

机构：

[1] UNIV TUBINGEN,INST ORGAN CHEM,D-72076 TUBINGEN,GERMANY

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1995年 / 7卷 / 01期

关键词：

ENANTIOMER SEPARATION; CAPILLARY ELECTROPHORESIS; CHIRAL BUFFER ADDITIVES; PROTEINS; CYCLODEXTRINS;

D O I：

10.1002/mcs.1220070103

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The direct enantiomeric analysis of six DNP-amino acids by capillary electrophoresis is reported. This study describes a comparison of several chiral buffer additives. Both the natural proteins BSA, AGP, ovomucoid and casein, and the cyclic oligosaccharides alpha-, beta-, and gamma-cyclodextrin were used as chiral selectors. For the first time, casein from goat milk was successfully applied for chiral discrimination. The influence of the modifier n-propanol on chiral discrimination is demonstrated with BSA as a buffer additive.

引用

页码：17 / 22

页数：6

共 32 条

[11] OPTICAL RESOLUTION BY ELECTROKINETIC CHROMATOGRAPHY USING OVOMUCOID AS A PSEUDO-STATIONARY PHASE [J].

ISHIHAMA, Y ;

ODA, Y ;

ASAKAWA, N ;

YOSHIDA, Y ;

SATO, T .

JOURNAL OF CHROMATOGRAPHY A, 1994, 666 (1-2) :193-201

[12]

JEANLOZ RW, 1966, GLYCOPROTEINS, P362

[13]

Konig W. A., 1992, GAS CHROMATOGRAPHIC

[14] CHIRAL SEPARATION BY CAPILLARY ELECTROPHORESIS [J].

KUHN, R ;

HOFFSTETTERKUHN, S .

CHROMATOGRAPHIA, 1992, 34 (9-10) :505-512

[15]

LEPRI L, 1992, Journal of Planar Chromatography - Modern TLC, V5, P175

[16] DIRECT SEPARATION OF ENANTIOMERS USING MULTIPLE-INTERACTION CHIRAL STATIONARY PHASES BASED ON THE MODIFIED BETA-CYCLODEXTRIN-BONDED STATIONARY PHASE [J].

LI, S ;

PURDY, WC .

JOURNAL OF CHROMATOGRAPHY, 1992, 625 (02) :109-120

[17] LIQUID-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF DINITROPHENYL AMINO-ACIDS USING A BETA-CYCLODEXTRIN-BONDED STATIONARY PHASE [J].

LI, S ;

PURDY, WC .

JOURNAL OF CHROMATOGRAPHY, 1991, 543 (01) :105-112

[18] PACKED-CAPILLARY ELECTROCHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF NEUTRAL AND ANIONIC COMPOUNDS USING BETA-CYCLODEXTRIN AS A CHIRAL SELECTOR - EFFECT OF OPERATING PARAMETERS AND COMPARISON WITH FREE-SOLUTION CAPILLARY ELECTROPHORESIS [J].

LI, S ;

LLOYD, DK .

JOURNAL OF CHROMATOGRAPHY A, 1994, 666 (1-2) :321-335

[19]

LOYD DK, 1994, S MOL CHIRALITY KYOT, P75

[20] SEPARATION OF ENANTIOMERS USING CELLULASE (CBH-I) SILICA AS A CHIRAL STATIONARY PHASE [J].

MARLE, I ;

ERLANDSSON, P ;

HANSSON, L ;

ISAKSSON, R ;

PETTERSSON, C ;

PETTERSSON, G .

JOURNAL OF CHROMATOGRAPHY, 1991, 586 (02) :233-248

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