EFFECTS OF PH AND HYDROXYPROPYL-BETA-CYCLODEXTRIN CONCENTRATION ON PEAK RESOLUTION IN THE CAPILLARY ELECTROPHORETIC SEPARATION OF THE ENANTIOMERS OF WEAK BASES

Weak base enantiomers were separated by capillary electrophoresis using hydroxypropyl p-cyclodextrin-containing background electrolytes. The multiple equilibria-based electrophoretic separation selectivity model (DID model) of enantiomers was used to analyze the results and determine the model parameters. The extended peak resolution equation of capillary electrophoresis has been applied to calculate the peak resolution surfaces as a function of the pH and the hydroxypropyl beta-cyclodextrin concentration of the background electrolyte, the dimensionless electroosmotic flow coefficient, and the effective portion of the applied potential. Combination of the DID selectivity model and the extended peak resolution equation allows the rational optimization of the operating conditions and the realization of separations previously deemed impossible.