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GROWTH AND SWAINSONINE PRODUCTION OF SWAINSONA-GALEGIFOLIA (ANDR) R-BR UNTRANSFORMED AND TRANSFORMED ROOT CULTURES
被引:10
作者:
ERMAYANTI, TM
[1
]
MCCOMB, JA
[1
]
OBRIEN, PA
[1
]
机构:
[1] MURDOCH UNIV,SCH BIOL & ENVIRONM SCI,MURDOCH,WA 6150,AUSTRALIA
关键词:
AGROBACTERIUM RHIZOGENES;
ROOT CULTURES;
SWAINSONA GALEGIFOLIA;
SWAINSONINE;
D O I:
10.1093/jxb/45.5.633
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
Untransformed and transformed root cultures of Swainsona galegifolia were established for swainsonine production. Transformed roots grew faster and produced higher swainsonine levels (62.3 mu g g(-1) DW) than untransformed roots (23.6 mu g g(-1) DW) or roots of intact plants (8.7 mu g g(-1) DW). Transformation of a number of plant genotypes using A. rhizogenes strain LBA 9402 showed that plant genotype influences swainsonine level in transformed roots but that a wide range of swainsonine levels can be induced by separate transformation events in the same genotype. Enhancement of swainsonine production was attempted by treatment with sugars and induction of polyploid roots.
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页码:633 / 639
页数:7
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