CROSS-CHALLENGE STUDIES IN RHESUS-MONKEYS EMPLOYING DIFFERENT INDIAN ISOLATES OF HEPATITIS-E VIRUS

被引：47

作者：

ARANKALLE, VA

CHADHA, MS

CHOBE, LP

NAIR, R

BANERJEE, K

机构：

[1] National Institute of Virology, Pune

来源：

JOURNAL OF MEDICAL VIROLOGY | 1995年 / 46卷 / 04期

关键词：

HEV ISOLATES; PCR; ANTI-HEV TITERS; CROSS-CHALLENGE;

D O I：

10.1002/jmv.1890460411

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study was to determine if rhesus monkeys infected with one isolate of hepatitis E virus (HEV) were immune to subsequent challenge with other isolates of the virus. Three epidemic and one sporadic Indian HEV isolates were employed in the study. The interval between primary inoculation and challenge varied from 1 year and 6 months to 2 years and 9 months. Evidence of HEV infection was ascertained by rise in serum alanine transaminase (ALT) levels and/or seroconversion to antibodies to HEV (anti-HEV), and the presence of HEV-RNA in the bile or faeces of the infected monkeys. No evidence for multiplication of virus in monkeys challenged with different HEV isolates was obtained. These results show that immunity generated by one isolate of HEV protects against different isolates of hepatitis E virus. (C) 1995 Wiley-Liss, Inc.

引用

页码：358 / 363

页数：6

共 22 条

[11] Huang CC, Nguyen D, Fernandez J, Yun KY, Fry KE, Bradley DW, Tam AW, Reyes GR, Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV), Virology, 191, pp. 550-558, (1992)
[12] Khuroo MS, Teli RT, Skidmore S, Sofi MA, Khuroo HI, Lncidence and severity of viral hepatitis in pregnancy, American Journal of Medicine, 70, pp. 252-255, (1981)
[13] Longer CF, Denny SL, Caudill JD, Mielde TA, Asher LVS, Myint KSA, Huang CC, Engler WF, Leduc JW, Binn LN, Ticehurst JR, Experimental hepatitis E: Pathogenesis in Cynomolgus macaques (Macaca fascicularis), The Journal of Infectious Diseases, 168, pp. 602-609, (1993)
[14] Purdy MA, McCaustland KA, Krawczynski K, Spelbring J, Reyes GR, Bradley DW, Preliminary evidence that a trp E‐HEV fusion protein protects cynomolgus macaques against challenge with wild‐type hepatitis E virus (HEV), Journal of Medical Virology, 41, pp. 90-94, (1993)
[15] Reyes GR, Purdy MA, Kim JP, Luk KC, Young LM, Fry KE, Bradley DW, Isolation of a cDNA from the virus responsible for enterically transmitted non‐A, non‐B hepatitis, Science, 247, pp. 1335-1339, (1990)
[16] Shrestha SM, Enteric non‐A, non‐B hepatitis in Nepal: Clinical and epidemiological observations, Viral hepatitis C, D&E, pp. 265-275, (1991)
[17] Tam AW, Smith MM, Guerra ME, Huang CC, Bradley DW, Fry KE, Reyes GR, Hepatitis E virus (HEV): Molecular cloning and sequencing of full‐length viral genome, Virology, 185, pp. 120-131, (1991)
[18] Tsarev SA, Emerson SU, Reyes GR, Tsareva TS, Legters U, Malik IA, Iqbal M, Purcell RH, Characterization of a prototype strain of hepatitis E virus, Proceedings of the National Academy of Sciences of the USA, 89, pp. 559-563, (1992)
[19] Tsarev SA, Tsareva TS, Emerson SU, Kapikian AZ, Ticehurst J, London W, Purcell RH, ELISA for antibody to hepatitis E virus (HEV) based on complete open‐reading frame‐2 protein expressed in insect cells: Identification of HEV infection in primates, The Journal of Infectious Diseases, 168, pp. 369-378, (1993)
[20] Uchida T, Win KM, Suzuki K, Gomatsu K, Ida F, Shikata T, Rikitica T, Mizuno K, Soe S, Myint H, Tin KM, Nakane K, Serial transmission of a putative causative virus of enterically‐transmitted non‐A, non‐B hepatitis to Macaca fascicularis and Macaca mulatta, Japanese Journal of Experimental Medicine, 60, pp. 13-22, (1990)

← 1 2 3 →