CLONING AND SEQUENCING SHOW THAT 4-HYDROXYBENZOATE HYDROXYLASE (POBA) IS REQUIRED FOR UPTAKE OF 4-HYDROXYBENZOATE IN RHIZOBIUM-LEGUMINOSARUM

被引：23

作者：

WONG, CM ^{[1
]}

DILWORTH, MJ ^{[1
]}

GLENN, AR ^{[1
]}

机构：

[1] MURDOCH UNIV, SCH BIOL & ENVIRONM SCI, NITROGEN FIXAT RES GRP, MURDOCH, WA 6150, AUSTRALIA

来源：

MICROBIOLOGY-SGM | 1994年 / 140卷

关键词：

RHIZOBIUM LEGUMINOSARUM; AROMATIC SUBSTRATE UPTAKE; 4-HYDROXYBENZOATE; SEQUENCE OF POBA;

D O I：

10.1099/00221287-140-10-2775

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutants of Rhizobium leguminosarum bv. viciae MNF300 and R. leguminosarum bv. trifolii WU95 unable to accumulate 4-hydroxybenzoate lack 4-hydroxybenzoate hydroxylase. The capacity of these mutants to take up and grow on 4-hydroxybenzoate was restored by a 2.0 kb EcoRI-PstI DNA fragment. This contained only one ORF which had over 60% DNA sequence similarity with the structural gene for 4-hydroxybenzoate hydroxylase (pobA) from Pseudomonas spp. and Acinetobacter. Reported effects of metabolic inhibitors and substrate analogues on the apparent uptake of 4-hydroxybenzoate have now been shown to be due to their direct effect on 4-hydroxybenzoate hydroxylase. We propose that uptake of 4-hydroxybenzoate is via a metabolic 'drag' mechanism dependent on the activity of the pobA gene product.

引用

页码：2775 / 2786

页数：12

共 40 条

[1]

Ausubel FM., 1988, CURRENT PROTOCOLS MO

[2] MUTATIONS THAT AFFECT ACTIVITY OF THE RHIZOBIUM-MELILOTI-TRPE(G) PROMOTER IN RHIZOBIUM-MELILOTI AND ESCHERICHIA-COLI [J].

BAE, YM ;

STAUFFER, GV .

JOURNAL OF BACTERIOLOGY, 1991, 173 (18) :5831-5836

[3] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].

BOYER, HW ;

ROULLAND.D .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&

[4]

BREWIN NJ, 1982, J GEN MICROBIOL, V128, P1817

[5] AMMONIA ASSIMILATION BY RHIZOBIUM CULTURES AND BACTEROIDS [J].

BROWN, CM ;

DILWORTH, MJ .

JOURNAL OF GENERAL MICROBIOLOGY, 1975, 86 (JAN) :39-48

[6]

CHEN YP, 1984, FEMS MICROBIOL LETT, V21, P201

[7] CLONING, NUCLEOTIDE SEQUENCING, AND EXPRESSION IN ESCHERICHIA-COLI OF A RHIZOBIUM-LEGUMINOSARUM GENE ENCODING A SYMBIOTICALLY REPRESSED OUTER-MEMBRANE PROTEIN [J].

DEMAAGD, RA ;

MULDERS, IHM ;

CREMERS, HCJC ;

LUGTENBERG, BJJ .

JOURNAL OF BACTERIOLOGY, 1992, 174 (01) :214-221

[8] GENETIC MANIPULATIONS IN RHIZOBIUM-MELILOTI UTILIZING 2 NEW TRANSPOSON TN5 DERIVATIVES [J].

DEVOS, GF ;

WALKER, GC ;

SIGNER, ER .

MOLECULAR & GENERAL GENETICS, 1986, 204 (03) :485-491

[9]

DILWORTH MJ, 1983, J GEN MICROBIOL, V129, P359

[10] EVOLUTIONARY DIVERGENCE OF POBA, THE STRUCTURAL GENE ENCODING P-HYDROXYBENZOATE HYDROXYLASE IN AN ACINETOBACTER-CALCOACETICUS STRAIN WELL-SUITED FOR GENETIC-ANALYSIS [J].

DIMARCO, AA ;

AVERHOFF, BA ;

KIM, EE ;

ORNSTON, LN .

GENE, 1993, 125 (01) :25-33

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