CLONING AND SEQUENCING SHOW THAT 4-HYDROXYBENZOATE HYDROXYLASE (POBA) IS REQUIRED FOR UPTAKE OF 4-HYDROXYBENZOATE IN RHIZOBIUM-LEGUMINOSARUM

被引：23

作者：

WONG, CM ^{[1
]}

DILWORTH, MJ ^{[1
]}

GLENN, AR ^{[1
]}

机构：

[1] MURDOCH UNIV, SCH BIOL & ENVIRONM SCI, NITROGEN FIXAT RES GRP, MURDOCH, WA 6150, AUSTRALIA

来源：

MICROBIOLOGY-SGM | 1994年 / 140卷

关键词：

RHIZOBIUM LEGUMINOSARUM; AROMATIC SUBSTRATE UPTAKE; 4-HYDROXYBENZOATE; SEQUENCE OF POBA;

D O I：

10.1099/00221287-140-10-2775

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutants of Rhizobium leguminosarum bv. viciae MNF300 and R. leguminosarum bv. trifolii WU95 unable to accumulate 4-hydroxybenzoate lack 4-hydroxybenzoate hydroxylase. The capacity of these mutants to take up and grow on 4-hydroxybenzoate was restored by a 2.0 kb EcoRI-PstI DNA fragment. This contained only one ORF which had over 60% DNA sequence similarity with the structural gene for 4-hydroxybenzoate hydroxylase (pobA) from Pseudomonas spp. and Acinetobacter. Reported effects of metabolic inhibitors and substrate analogues on the apparent uptake of 4-hydroxybenzoate have now been shown to be due to their direct effect on 4-hydroxybenzoate hydroxylase. We propose that uptake of 4-hydroxybenzoate is via a metabolic 'drag' mechanism dependent on the activity of the pobA gene product.

引用

页码：2775 / 2786

页数：12

共 40 条

[31] CLONING AND EXPRESSION OF ACINETOBACTER-CALCOACETICUS CATBCDE GENES IN PSEUDOMONAS-PUTIDA AND ESCHERICHIA-COLI [J].

SHANLEY, MS ;

NEIDLE, EL ;

PARALES, RE ;

ORNSTON, LN .

JOURNAL OF BACTERIOLOGY, 1986, 165 (02) :557-563

[32]

SMITH I, 1960, CHROMATOGR ELECTROPH, V1, P291

[33]

STANIER RY, 1973, ADV MICROB PHYSL, V9, P59

[34] MOLECULAR CHARACTERIZATION OF CLONED AVIRULENCE GENES FROM RACE-0 AND RACE-1 OF PSEUDOMONAS-SYRINGAE PV GLYCINEA [J].

STASKAWICZ, B ;

DAHLBECK, D ;

KEEN, N ;

NAPOLI, C .

JOURNAL OF BACTERIOLOGY, 1987, 169 (12) :5789-5794

[35]

THAYER JR, 1982, J GEN MICROBIOL, V128, P1749

[36]

VANBERKEL W, 1992, EUR J BIOCHEM, V210, P411

[37] GENETICS OF DISSIMILARITY PATHWAYS IN PSEUDOMONAS [J].

WHEELIS, ML .

ANNUAL REVIEW OF MICROBIOLOGY, 1975, 29 :505-524

[38] EVIDENCE FOR 2 UPTAKE SYSTEMS IN RHIZOBIUM-LEGUMINOSARUM FOR HYDROXY-AROMATIC COMPOUNDS METABOLIZED BY THE 3-OXOADIPATE PATHWAY [J].

WONG, CM ;

DILWORTH, MJ ;

GLENN, AR .

ARCHIVES OF MICROBIOLOGY, 1991, 156 (05) :385-391

[39] IMPROVED M13 PHAGE CLONING VECTORS AND HOST STRAINS - NUCLEOTIDE-SEQUENCES OF THE M13MP18 AND PUC19 VECTORS [J].

YANISCHPERRON, C ;

VIEIRA, J ;

MESSING, J .

GENE, 1985, 33 (01) :103-119

[40]

YEH WK, 1981, J BIOL CHEM, V256, P1565

← 1 2 3 4 →