SEPARATION OF R-HIRUDIN FROM SIMILAR SUBSTANCES BY CAPILLARY ELECTROPHORESIS

被引：13

作者：

DETTE, C ^{[1
]}

WATZIG, H ^{[1
]}

机构：

[1] UNIV WURZBURG,INST PHARM & FOOD CHEM,D-97074 WURZBURG,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 700卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01261-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The thrombin inhibitor r-hirudin is a peptide of 65 amino acids and with a pi of 4.4. Capillary electrophoresis (CE) was used to separate r-hirudin from seven closely related substances which may be found as by-products or from degradation. Possibly two of these substances differ only in an isoaspartyl instead of an aspartyl binding. A baseline separation was possible with an acetate buffer (pH 4.4, 60 mM) containing 0.3% (m/m) PEG 20 000 and 0.1 mM Zn2+. The possibilities to prevent wall adsorption are discussed.

引用

页码：89 / 94

页数：6

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