EVALUATION OF PHENOBARBITAL BETA-NAPHTHOFLAVONE AS AN ALTERNATIVE S9-INDUCTION REGIME TO AROCLOR-1254 IN THE RAT FOR USE IN IN-VITRO GENOTOXICITY ASSAYS

被引：33

作者：

CALLANDER, RD ^{[1
]}

MACKAY, JM ^{[1
]}

CLAY, P ^{[1
]}

ELCOMBE, CR ^{[1
]}

ELLIOTT, BM ^{[1
]}

机构：

[1] ZENECA CENT TOXICOL LAB,MACCLESFIELD SK10 4TJ,CHESHIRE,ENGLAND

来源：

MUTAGENESIS | 1995年 / 10卷 / 06期

关键词：

D O I：

10.1093/mutage/10.6.517

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A series of bacterial mutation, mammalian cell (L5178Y) gene mutation and in vitro cytogenetic assays were performed to compare the efficacy of using S9 fractions prepared from rats induced with a combination of phenobarbital (PB) and beta-naphthoflavone (beta NF), with So fractions from rats treated with the general enzyme inducer Aroclor 1254. Although some quantitative differences in the magnitudes of the mutagenic/clastogenic effects were observed between the two induction regimes, no qualitative differences were observed. The use of a combined PB/beta NF induction regime using oral dosing is therefore considered to be a suitable substitute for Aroclor 1254.

引用

页码：517 / 522

页数：6

共 12 条

[1] METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].

AMES, BN ;

MCCANN, J ;

YAMASAKI, E .

MUTATION RESEARCH, 1975, 31 (06) :347-363

[2] INDUCTION OF CYTOCHROME-P-450 IN CULTURED RAT HEPATOCYTES - THE HETEROGENEOUS LOCALIZATION OF SPECIFIC ISOENZYMES USING IMMUNOCYTOCHEMISTRY [J].

BARS, RG ;

MITCHELL, AM ;

WOLF, CR ;

ELCOMBE, CR .

BIOCHEMICAL JOURNAL, 1989, 262 (01) :151-158

[3] MUTAGENICITY OF POLYCYCLIC AROMATIC-HYDROCARBONS [J].

BROOKES, P .

MUTATION RESEARCH, 1977, 39 (3-4) :257-284

[4] ETHOXYPHENOXAZONES, PENTOXYPHENOXAZONES, AND BENZYLOXYPHENOXAZONES AND HOMOLOGS - A SERIES OF SUBSTRATES TO DISTINGUISH BETWEEN DIFFERENT INDUCED CYTOCHROMES-P-450 [J].

BURKE, MD ;

THOMPSON, S ;

ELCOMBE, CR ;

HALPERT, J ;

HAAPARANTA, T ;

MAYER, RT .

BIOCHEMICAL PHARMACOLOGY, 1985, 34 (18) :3337-3345

[5] OBSERVED CONVERGENCE OF THE SALMONELLA PLATE AND PREINCUBATION ASSAYS WHEN EMPLOYING VARYING LEVELS OF S9 [J].

CALLANDER, RD .

MUTAGENESIS, 1986, 1 (06) :439-443

[6]

Clare C., 1989, BIBRA B, V28, P111

[7]

CLAY P, 1990, MUTAGENESIS, V5, P45

[8] ALTERNATIVES TO AROCLOR 1254-INDUCED S9 IN INVITRO GENOTOXICITY ASSAYS [J].

ELLIOTT, BM ;

COMBES, RD ;

ELCOMBE, CR ;

GATEHOUSE, DG ;

GIBSON, GG ;

MACKAY, JM ;

WOLF, RC .

MUTAGENESIS, 1992, 7 (03) :175-177

[9]

Gatehouse D., 1990, BASIC MUTAGENICITY T, P13

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

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