THE PLASMA-MEMBRANE H+-ATPASE FROM HIGHER-PLANTS - FUNCTIONAL RECONSTITUTION INTO LIPOSOMES AND ITS REGULATION BY PHOSPHOLIPIDS

被引：24

作者：

KASAMO, K ^{[1
]}

SAKAKIBARA, Y ^{[1
]}

机构：

[1] NATL FOOD RES INST,TSUKUBA,IBARAKI 305,JAPAN

来源：

PLANT SCIENCE | 1995年 / 111卷 / 02期

关键词：

RECONSTITUTION; PLASMA MEMBRANE; H+-ATPASE; LIPOSOMES; PROTON PUMPING;

D O I：

10.1016/0168-9452(95)04224-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the past 6 years, molecular and genetic studies of plant plasma membrane H+-ATPase (PMHA) have been achieved to elucidate the relation between its gene structure and the expression of the gene. The reconstitution technique is a useful tool, so that functional reconstitution of the PMHA into liposomes has been successfully carried out to directly demonstrate the function of the membrane protein. During purification and reconstitution, PMHA activity and proton pumping were found to be regulated by phospholipids. This review summarizes currently available information concerning the physiological functions, molecular genetics, enzyme structure and functional reconstitution of the plant PMHA.

引用

页码：117 / 131

页数：15

共 136 条

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