CHARACTERIZATION OF A CHIMERIC HD(3)/D(2) DOPAMINE RECEPTOR EXPRESSED IN CHO CELLS

被引:48
作者
MCALLISTER, G [1 ]
KNOWLES, MR [1 ]
PATEL, S [1 ]
MARWOOD, R [1 ]
EMMS, F [1 ]
SEABROOK, GR [1 ]
GRAZIANO, M [1 ]
BORKOWSKI, D [1 ]
HEY, PJ [1 ]
FREEDMAN, SB [1 ]
机构
[1] MERCK SHARP & DOHME LTD,RAHWAY,NJ 07065
关键词
DOPAMINE RECEPTOR; SIGNAL TRANSDUCTION; FUNCTIONAL COUPLING;
D O I
10.1016/0014-5793(93)81537-A
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The D2 dopamine receptor is known to be functionally coupled when expressed in CHO cells, whereas the effector systems for the D3 dopamine receptor remain unclear. A chimeric, human D3/D2 receptor (hD3/D2) was constructed containing the third intracellular loop region of the D2 receptor. CHO cells stably expressing the D2, D3, or hD3/D2 receptors were created and the pharmacology of the receptors was examined. The chimeric hD3/D2 receptor retained D3-like affinities for dopaminergic ligands. However, in contrast to the D2 receptor neither the D3 receptor nor the hD3/D2 receptor could functionally couple to the adenylate cyclase or arachidonic acid release mechanisms.
引用
收藏
页码:81 / 86
页数:6
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