CHEMILUMINESCENT ASSAY FOR GLYCEROL IN WINE USING FLOW-INJECTION

被引：18

作者：

PUCHADES, R ^{[1
]}

LEMIEUX, L ^{[1
]}

SIMARD, RE ^{[1
]}

机构：

[1] UNIV LAVAL,DEPT SCI & TECHNOL ALIMENTS,STELA,RECH GRP,QUEBEC CITY G1K 7P4,QUEBEC,CANADA

来源：

JOURNAL OF FOOD SCIENCE | 1991年 / 56卷 / 04期

关键词：

D O I：

10.1111/j.1365-2621.1991.tb14650.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A flow-injection method was developed for the enzymatic determination of glycerol in wines using a reactor containing immobilized glycerokinase and glycerol-3'-phosphate oxidase. Hydrogen peroxide from the reaction was monitored by chemiluminescence after reaction with an alkaline reagent containing luminol and hexacyanoferrate (III). Calibration curves were linear between 25 mg/L and 250 mg/L of glycerol for injected volume 29-mu-L. The method gave satisfactory results compared with a gas-chromatographic method. No sample pretreatment was necessary and a sampling frequency of 30/hr could be achieved.

引用

页码：1097 / 1100

页数：4

共 43 条

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