CHEMILUMINESCENT ASSAY FOR GLYCEROL IN WINE USING FLOW-INJECTION

A flow-injection method was developed for the enzymatic determination of glycerol in wines using a reactor containing immobilized glycerokinase and glycerol-3'-phosphate oxidase. Hydrogen peroxide from the reaction was monitored by chemiluminescence after reaction with an alkaline reagent containing luminol and hexacyanoferrate (III). Calibration curves were linear between 25 mg/L and 250 mg/L of glycerol for injected volume 29-mu-L. The method gave satisfactory results compared with a gas-chromatographic method. No sample pretreatment was necessary and a sampling frequency of 30/hr could be achieved.