MOLECULAR AND PHYSICAL ORGANIZATION OF HIGHLY REPETITIVE, UNDERMETHYLATED DNA FROM PENNISETUM-GLAUCUM

被引：42

作者：

KAMM, A

SCHMIDT, T

HESLOPHARRISON, JS

机构：

[1] Karyobiology Group, Department of Cell Biology, John Innes Centre for Plant Science Research, Colney Lane, NR4 7UJ, Norwich

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 244卷 / 04期

关键词：

PENNISETUM GLAUCUM; SATELLITE DNA; IN SITU HYBRIDIZATION; CENTROMERIC HETEROCHROMATIN; METHYLATION;

D O I：

10.1007/BF00286694

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A HaeIII monomer of a repetitive DNA family from Pennisetum glaucum (L.) R. Br. cv. Massue has been cloned and characterized. The repeat is 137 bp long and is organized in head-to-tail orientation in tandem arrays. The HaeIII monomer contains 55% A + T residues. The distribution of this highly repetitive sequence in different Pennisetum species and in other cereals was investigated. The HaeIII satellite is present in all Pennisetum species investigated but absent from other genera examined. In situ hybridization revealed a centromeric localization of this sequence on all seven chromosome pairs and indicated chromosome-specific differences in copy number. Methylation was investigated by comparative restriction enzyme analysis (MspI/HpaII) which showed a greater extent of methylation of the internal C of the enzyme recognition site 5'-CCGG. A South-Western analysis, using an anti-methylcytosine antibody to examine the methylation status in P. glaucum confirmed that the sequence is not highly methylated.

引用

页码：420 / 425

页数：6

共 27 条

[1] ISOLATION AND CHARACTERIZATION OF GENOME-SPECIFIC DNA-SEQUENCES IN TRITICEAE SPECIES [J].

ANAMTHAWATJONSSON, K ;

HESLOPHARRISON, JS .

MOLECULAR AND GENERAL GENETICS, 1993, 240 (02) :151-158

[2] MOLECULAR DESCRIPTION OF TELOMERIC HETEROCHROMATIN IN SECALE SPECIES [J].

BEDBROOK, JR ;

JONES, J ;

ODELL, M ;

THOMPSON, RD ;

FLAVELL, RB .

CELL, 1980, 19 (02) :545-560

[3] AMPLIFICATION OF REARRANGED REPEATED DNA-SEQUENCES IN CEREAL PLANTS [J].

BEDBROOK, JR ;

ODELL, M ;

FLAVELL, RB .

NATURE, 1980, 288 (5787) :133-137

[4] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].

BENNETT, MD ;

SMITH, JB ;

HESLOPHARRISON, JS .

PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1982, 216 (1203) :179-199

[5] DISTRIBUTION AND ORGANIZATION OF A TANDEMLY REPEATED 352-BP SEQUENCE IN THE ORYZAE FAMILY [J].

DEKOCHKO, A ;

KIEFER, MC ;

CORDESSE, F ;

REDDY, AS ;

DELSENY, M .

THEORETICAL AND APPLIED GENETICS, 1991, 82 (01) :57-64

[6] IDENTICAL POLYPYRIMIDINE-POLYPURINE SATELLITE DNAS IN WHEAT AND BARLEY [J].

DENNIS, ES ;

GERLACH, WL ;

PEACOCK, WJ .

HEREDITY, 1980, 44 (JUN) :349-+

[7] THE MOLECULAR CHARACTERIZATION AND ORGANIZATION OF PLANT CHROMOSOMAL DNA-SEQUENCES [J].

FLAVELL, R .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1980, 31 :569-596

[8] THE CHROMOSOMAL DISTRIBUTION OF CLONED HIGHLY REPETITIVE SEQUENCES FROM HEXAPLOID WHEAT [J].

HUTCHINSON, J ;

LONSDALE, DM .

HEREDITY, 1982, 48 (JUN) :371-376

[9] ORIGIN OF THE MAIN CLASS OF REPETITIVE DNA WITHIN SELECTED PENNISETUM SPECIES [J].

INGHAM, LD ;

HANNA, WW ;

BAIER, JW ;

HANNAH, LC .

MOLECULAR & GENERAL GENETICS, 1993, 238 (03) :350-356

[10] MOLECULAR AND CYTOLOGICAL CHARACTERIZATION OF REPETITIVE DNA-SEQUENCES IN BRASSICA [J].

IWABUCHI, M ;

ITOH, K ;

SHIMAMOTO, K .

THEORETICAL AND APPLIED GENETICS, 1991, 81 (03) :349-355

← 1 2 3 →