A PHYSICAL AND GENETIC-MAP OF THE SPIROPLASMA-CITRI GENOME

被引：49

作者：

YE, FC

LAIGRET, F

WHITLEY, JC

CITTI, C

FINCH, LR

CARLE, P

RENAUDIN, J

BOVE, JM

机构：

[1] LAB BIOL CELLULAIRE & MOLEC,F-33883 VILLENAVE DORNON,FRANCE

[2] UNIV MELBOURNE,RUSSELL GRIMWADE SCH BIOCHEM,PARKVILLE,VIC 3052,AUSTRALIA

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 07期

关键词：

D O I：

10.1093/nar/20.7.1559

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A physical and genetic map of the Spiroplasma citri genome has been constructed using several restriction enzymes and pulsed field gel electrophoresis. A number of genes were subsequently localized on the map by the use of appropriate probes. The genome size of the spiroplasma estimated from restriction fragments is close to 1780 kbp, the largest of all Mollicutes studied so far. It contains multisite insertions of Spiroplasma virus 1 (SpV1) sequences. The physical and genetic map of the S. citri genome shares several features with that of other Mollicutes, especially those in the Mycoplasma mycoides cluster. This supports the finding that S. citri and these Mycoplasma spp. are phylogenetically related.

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页码：1559 / 1565

页数：7

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