LOCALIZATION OF DYSTROPHIN IN THE PURKINJE-CELLS OF NORMAL MICE

被引：35

作者：

HUARD, J ^{[1
]}

TREMBLAY, JP ^{[1
]}

机构：

[1] UNIV LAVAL,HOP ENFANT JESUS,NEUROBIOL LAB,1401,18E RUE,QUEBEC CITY G1J 1Z4,QUEBEC,CANADA

来源：

NEUROSCIENCE LETTERS | 1992年 / 137卷 / 01期

基金：

英国医学研究理事会;

关键词：

DYSTROPHIN; NORMAL MOUSE; DUCHENNE MUSCULAR DYSTROPHY; PURKINJE CELL; MDX MOUSE; IMMUNOCYTOCHEMISTRY; IMMUNOBLOTTING;

D O I：

10.1016/0304-3940(92)90309-U

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A monoclonal antibody that reacts with a mid rod fragment of dystrophin was used to localize this protein in the central nervous system (CNS). Due to a low abundance of dystrophin in the CNS, an immunoperoxidase reaction amplified with a biotin-avidin system was used. All Purkinje cells in normal mice were dystrophin positive while the mdx mouse cerebellum was completely devoid of reaction. Dystrophin staining was present in the soma and dendrites of Purkinje cells but not in their axons. This uniform dystrophin labelling in the normal mouse Purkinje cells indicates that this protein is not only localized in synaptic contact regions of the CNS.

引用

页码：105 / 108

页数：4

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