DIFFERENTIATION OF PSEUDOMONAS-AERUGINOSA PILI BASED ON SEQUENCE AND B-CELL EPITOPE ANALYSES

被引：39

作者：

CASTRIC, PA ^{[1
]}

DEAL, CD ^{[1
]}

机构：

[1] WALTER REED ARMY INST RES, DEPT BACTERIAL DIS, WASHINGTON, DC 20307 USA

来源：

INFECTION AND IMMUNITY | 1994年 / 62卷 / 02期

关键词：

D O I：

10.1128/IAI.62.2.371-376.1994

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping immobilized peptides representing the pilin primary structure, with antipilin monoclonal antibodies revealed several B-cell determinants grouped near the carboxyl terminus of P. aeruginosa 1244 pilin. One determinant was found to reside near the pilin constant region. These determinants were found associated with the pill of 31 of 95 P. aeruginosa clinical isolates.

引用

页码：371 / 376

页数：6

共 36 条

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