STRUCTURE OF THE GENE FOR HUMAN URACIL-DNA GLYCOSYLASE AND ANALYSIS OF THE PROMOTER FUNCTION

被引：24

作者：

HAUG, T ^{[1
]}

SKORPEN, F ^{[1
]}

LUND, H ^{[1
]}

KROKAN, HE ^{[1
]}

机构：

[1] UNIV TRONDHEIM,UNIGEN,CTR MOLEC BIOL,N-7005 TRONDHEIM,NORWAY

来源：

FEBS LETTERS | 1994年 / 353卷 / 02期

关键词：

URACIL-DNA GLYCOSYLASE; GENE EXPRESSION; PROMOTER FUNCTION;

D O I：

10.1016/0014-5793(94)01042-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene for human uracil-DNA glycosylase (UNG) contains 4 exons and has an approximate size of 13 kb. The promoter is very GC rich and lacks a TATA box. Nested deletions of the promoter demonstrated that two SP1 elements and a putative c-MYC element proximal to the transcription initiation region were sufficient to support some 27% of the promoter activity, while a clone that in addition contained the elements E2F/SP1/CCAAT increased expression to almost 90% of the full-length construct. A region upstream of these elements appears to exert a negative control function.

引用

页码：180 / 184

页数：5

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