DETECTION OF ROCHALIMAEA-HENSELAE DNA IN SPECIMENS FROM CAT-SCRATCH DISEASE PATIENTS BY PCR

被引:243
作者
ANDERSON, B [1 ]
SIMS, K [1 ]
REGNERY, R [1 ]
ROBINSON, L [1 ]
SCHMIDT, MJ [1 ]
GORAL, S [1 ]
HAGER, C [1 ]
EDWARDS, K [1 ]
机构
[1] VANDERBILT UNIV,DEPT PEDIAT,NASHVILLE,TN 37232
关键词
D O I
10.1128/JCM.32.4.942-948.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A PCR assay was developed by using degenerate primers that allow amplification of a 414-bp fragment of DNA from the rickettsia-like organisms Rochalimaea henselae and R. quintana. Internal oligonucleotides were used as hybridization probes, permitting rapid differentiation of these two Rochalimaea species. DNAs from 12 different isolates of R. henselae were amplified with the PCR primers, and the resulting 414-bp PCR product hybridized only with the R. henselae-specific probe. DNAs from four different isolates of R. quintana were amplified and produced a PCR product of the same size that hybridized only with the R. quintana-specific probe. DNAs from isolates of R. elizabethae, R. vinsonii, Bartonella bacilliformis, and Afipia felis failed to amplify the 414-bp fragment in the PCR assay. This two-step assay was applied to DNAs extracted from 16 fresh (unfixed) lymph node biopsy specimens and nine aspirates from patients with clinical cat scratch disease (CSI)) to assay for the presence of R. henselae or R. quintana DNA in these samples. Twenty-one (84%) of 25 lymph node samples from CSD patients were positive for R. henselae, while none were positive for R. quintana. The characteristic 414-bp fragment was not amplified from eight lymph node tissue samples from non-CSD cases. These results provide evidence that R. henselae, and not R. quintana, plays the central role in the etiology of CSD.
引用
收藏
页码:942 / 948
页数:7
相关论文
共 27 条
[21]   THE AGENT OF BACILLARY ANGIOMATOSIS - AN APPROACH TO THE IDENTIFICATION OF UNCULTURED PATHOGENS [J].
RELMAN, DA ;
LOUTIT, JS ;
SCHMIDT, TM ;
FALKOW, S ;
TOMPKINS, LS .
NEW ENGLAND JOURNAL OF MEDICINE, 1990, 323 (23) :1573-1580
[22]   A NEWLY RECOGNIZED FASTIDIOUS GRAM-NEGATIVE PATHOGEN AS A CAUSE OF FEVER AND BACTEREMIA [J].
SLATER, LN ;
WELCH, DF ;
HENSEL, D ;
COODY, DW .
NEW ENGLAND JOURNAL OF MEDICINE, 1990, 323 (23) :1587-1593
[23]   THE EPIDEMIOLOGY OF BACILLARY ANGIOMATOSIS AND BACILLARY PELIOSIS [J].
TAPPERO, JW ;
MOHLEBOETANI, J ;
KOEHLER, JE ;
SWAMINATHAN, B ;
BERGER, TG ;
LEBOIT, PE ;
SMITH, LL ;
WENGER, JD ;
PINNER, RW ;
KEMPER, CA ;
REINGOLD, AL .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1993, 269 (06) :770-775
[24]   ROCHALIMAEA-HENSELAE SP-NOV, A CAUSE OF SEPTICEMIA, BACILLARY ANGIOMATOSIS, AND PARENCHYMAL BACILLARY PELIOSIS [J].
WELCH, DF ;
PICKETT, DA ;
SLATER, LN ;
STEIGERWALT, AG ;
BRENNER, DJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (02) :275-280
[25]   BACTEREMIA DUE TO ROCHALIMAEA-HENSELAE IN A CHILD - PRACTICAL IDENTIFICATION OF ISOLATES IN THE CLINICAL LABORATORY [J].
WELCH, DF ;
HENSEL, DM ;
PICKETT, DA ;
JOAQUIN, VHS ;
ROBINSON, A ;
SLATER, LN .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (09) :2381-2386
[26]   ALLELE-SPECIFIC ENZYMATIC AMPLIFICATION OF BETA-GLOBIN GENOMIC DNA FOR DIAGNOSIS OF SICKLE-CELL ANEMIA [J].
WU, DY ;
UGOZZOLI, L ;
PAL, BK ;
WALLACE, RB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2757-2760
[27]   CAT-SCRATCH DISEASE IN CONNECTICUT - EPIDEMIOLOGY, RISK-FACTORS, AND EVALUATION OF A NEW DIAGNOSTIC-TEST [J].
ZANGWILL, KM ;
HAMILTON, DH ;
PERKINS, BA ;
REGNERY, RL ;
PLIKAYTIS, BD ;
HADLER, JL ;
CARTTER, ML ;
WENGER, JD .
NEW ENGLAND JOURNAL OF MEDICINE, 1993, 329 (01) :8-13