ANALYSIS OF GLYCOSPHINGOLIPIDS BY FLUOROPHORE-ASSISTED CARBOHYDRATE ELECTROPHORESIS USING CERAMIDE GLYCANASE FROM MERCENARIA-MERCENARIA

被引：24

作者：

BASU, SS ^{[1
]}

DASTGHEIBHOSSEINI, S ^{[1
]}

HOOVER, G ^{[1
]}

LI, ZX ^{[1
]}

BASU, S ^{[1
]}

机构：

[1] UNIV NOTRE DAME,DEPT CHEM & BIOCHEM,NOTRE DAME,IN 46556

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 222卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1484

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyacrylamide gel electrophoresis of fluorophore-labeled saccharides is a simple and sensitive separation method for glycan analysis. This method requires an aldehydic reducing carbon on the saccharide in order to react with the amine group of the fluorophore (8-aminonaphthalene- 1,3,6-trisulfonic acid or 2-aminoacridone). We have used exoglycosidase-free ceramide glycanase from hard-shelled clam to expose the reducing terminal of the glycan in glycosphingolipid by cleaving the linkage between the glycan and the ceramide. The released glycan was used for fluorophore-assisted carbohydrate electrophoresis, in order to qualitatively determine its monosaccharide composition, chain length, and glycosidic linkages using linkage-specific glycosidases, including a beta 1-3,4 galactosidase from clam. (C) 1994 Academic Press, Inc.

引用

页码：270 / 274

页数：5

共 21 条

[11] THE ANALYSIS OF FLUOROPHORE-LABELED GLYCANS BY HIGH-RESOLUTION POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

JACKSON, P .

ANALYTICAL BIOCHEMISTRY, 1994, 216 (02) :243-252

[12]

JACKSON P, 1994, METHOD ENZYMOL, V230, P250

[13] ANIMAL GLYCOSPHINGOLIPIDS AS MEMBRANE ATTACHMENT SITES FOR BACTERIA [J].

KARLSSON, KA .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :309-350

[14] OCCURRENCE OF CERAMIDE-GLYCANASE IN THE EARTHWORM, LUMBRICUS-TERRESTRIS [J].

LI, YT ;

ISHIKAWA, Y ;

LI, SC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 149 (01) :167-172

[15]

LI Z, 1994, FASEB J, V8, pA1439

[16] ELAM-1-DEPENDENT CELL-ADHESION TO VASCULAR ENDOTHELIUM DETERMINED BY A TRANSFECTED HUMAN FUCOSYL-TRANSFERASE CDNA [J].

LOWE, JB ;

STOOLMAN, LM ;

NAIR, RP ;

LARSEN, RD ;

BERHEND, TL ;

MARKS, RM .

CELL, 1990, 63 (03) :475-484

[17] ELAM-1 MEDIATES CELL-ADHESION BY RECOGNITION OF A CARBOHYDRATE LIGAND, SIALYL-LEX [J].

PHILLIPS, ML ;

NUDELMAN, E ;

GAETA, FCA ;

PEREZ, M ;

SINGHAL, AK ;

HAKOMORI, SI ;

PAULSON, JC .

SCIENCE, 1990, 250 (4984) :1130-1132

[18] ELECTROSPRAY IONIZATION MASS-SPECTROMETRY - DECONVOLUTION BY AN ENTROPY-BASED ALGORITHM [J].

REINHOLD, BB ;

REINHOLD, VN .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1992, 3 (03) :207-215

[19]

STACK RJ, 1991, GLYCOCONJ J, V8, P249

[20]

WESTERVELT C, 1989, GLYCOCONJUGATE J, V6, P386

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