LIGHT ADDRESSABLE POTENTIOMETRIC BIOSENSOR FOR THE DETECTION OF ANTICHOLINESTERASES

被引：36

作者：

ROGERS, KR ^{[1
]}

FOLEY, M ^{[1
]}

ALTER, S ^{[1
]}

KOGA, P ^{[1
]}

ELDEFRAWI, M ^{[1
]}

机构：

[1] ENVIRONM TECHNOL GRP INC,BALTIMORE,MD 21039

来源：

ANALYTICAL LETTERS | 1991年 / 24卷 / 02期

关键词：

LAPS; ANTICHOLINESTERASE; BIOSENSOR; ACETYLCHO LINESTERASE;

D O I：

10.1080/00032719108052896

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Biotinylated acetylcholinesterase (AChE) from Eel was immobilized to a biotinylated cellulose nitrate membrane via a streptavidin bridge. Using a light addressable potentiometric sensor (LAPS), enzyme activity was determined as a function of substrate concentration (i.e. acetylcholine) and amount of enzyme immobilized to the membrane. This biosensor was also evaluated for its potential to detect organophosphate (OP) anticholinesterases (antiChEs).

引用

页码：191 / 198

页数：8

共 20 条

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