THIMEROSAL CAUSES CALCIUM OSCILLATIONS AND SENSITIZES CALCIUM-INDUCED CALCIUM RELEASE IN UNFERTILIZED HAMSTER EGGS

被引：138

作者：

SWANN, K

机构：

[1] MRC Experimental Embryology and Teratology Unit. St. George's Hospital Medical School, London, SW17 0RE, Cranmer Terrace

来源：

FEBS LETTERS | 1991年 / 278卷 / 02期

关键词：

CALCIUM ION RELEASE; HAMSTER EGG; CALCIUM ACTIVATED POTASSIUM CONDUCTANCE; SULFHYDRYL REAGENT;

D O I：

10.1016/0014-5793(91)80110-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcium-induced-calcium-release (CICR) was assayed in unfertilized golden hamster eggs by injecting Ca2+ and monitoring Ca2+ -dependent hyperpolarizing responses (HRs) and Ca2+ -sensitive fluo-3 fluorescence. Incubating eggs in the sulfhydryl reagent thimerosal caused [Ca2+]i oscillations as monitored by Ca2+ -dependent HRs and decreased approximately 10-fold the Ca2+ injection current required to generate an HR and cause a large intracellular Ca2+ increase. Thimerosal also enhanced the sensitivity of eggs to Ca2+ injection in a calcium-free medium. The effects of thimerosal on CICR were prevented by dithiothreitol and were not mimicked by injecting inositol 1,4,5-trisphosphate. The data suggest that thimerosal may be an alternative agent for studying CICR in caffeine-insensitive cells.

引用

页码：175 / 178

页数：4

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