ELECTROCHEMICAL DETECTION AS AN ALTERNATIVE TO UV IN RP-HPLC PEPTIDE-MAPPING

被引：29

作者：

CHEN, L ^{[1
]}

KRULL, IS ^{[1
]}

机构：

[1] NORTHEASTERN UNIV,DEPT CHEM,BOSTON,MA 02115

来源：

ELECTROANALYSIS | 1994年 / 6卷 / 01期

关键词：

REVERSED-PHASE HPLC; PHOTOLYTIC DERIVATIZATION; PEPTIDE MAPPING; AMPEROMETRIC DETECTION; AMINO ACIDS;

D O I：

10.1002/elan.1140060103

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article describes the possibility of electrochemical (EC) detection as an alternative to ultraviolet (UV) detection doing peptide mapping. The high sensitivity and selectivity of the electrochemical detection can be applied to determine some digested peptides. This approach demonstrated that peptides containing electroactive amino acids can be determined by EC detection. Furthermore, upon irradiation, nonelectroactive amino acids and peptides can be made electroactive, thereby increasing the sensitivity and selectivity of peptide mapping. A variety of effects are discussed to help in understanding the possibility of this useful approach.

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页码：1 / 8

页数：8

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