27-HYDROXYCHOLESTEROL MODULATION OF LOW-DENSITY-LIPOPROTEIN METABOLISM IN CULTURED HUMAN HEPATIC AND EXTRAHEPATIC CELLS

被引：10

作者：

BELLOSTA, S ^{[1
]}

CORSINI, A ^{[1
]}

BERNINI, F ^{[1
]}

GRANATA, A ^{[1
]}

DIDONI, G ^{[1
]}

MAZZOTTI, M ^{[1
]}

FUMAGALLI, R ^{[1
]}

机构：

[1] UNIV MILAN,INST PHARMACOL SCI,I-20122 MILAN,ITALY

来源：

FEBS LETTERS | 1993年 / 332卷 / 1-2期

关键词：

OXYSTEROL; LDL RECEPTOR; HUMAN HEPATOMA CELL LINE HEP G2; HUMAN ENDOTHELIAL CELL; HUMAN FIBROBLAST;

D O I：

10.1016/0014-5793(93)80495-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

27-Hydroxycholesterol**, 25-hydroxycholesterol and cholesterol suppressed LDL uptake and degradation in human extrahepatic and hepatic cell lines in a concentration-dependent manner. Cholesterol was the least potent, and the inhibitory effect of oxysterols was more pronounced in skin fibroblasts and in endothelial cell line EAhy 926 than in hepatoma HepG2 cells. Shorter incubations were required for oxysterols to achieve 50% inhibition of LDL uptake and degradation in all cultured cells. The inhibition of LDL catabolism in extrahepatic cells by 27-hydroxycholesterol occurred at concentrations close to those observed in human plasma (0.2-0.6 muM). The results support a possible role of 27-hydroxycholesterol, a physiological oxysterol, in the regulation of cellular cholesterol homeostasis in non-hepatic tissues.

引用

页码：115 / 118

页数：4

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