BIOCONVERSION OF N-OCTANE TO OCTANOIC-ACID BY A RECOMBINANT ESCHERICHIA-COLI CULTURED IN A 2-LIQUID PHASE BIOREACTOR

被引：71

作者：

FAVREBULLE, O ^{[1
]}

SCHOUTEN, T ^{[1
]}

KINGMA, J ^{[1
]}

WITHOLT, B ^{[1
]}

机构：

[1] STATE UNIV GRONINGEN, GRONINGEN BIOTECHNOL CTR, NIJENBORGH 16, 9747 AG GRONINGEN, NETHERLANDS

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 04期

关键词：

D O I：

10.1038/nbt0491-367

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The alk genes from the catabolic OCT plasmid of Pseudomonas oleovorans, which encode the enzymes involved in the oxidation of n-alkanes to carboxylic acids, were introduced into E. coli W3110. The resulting recombinant converts n-octane in a two-liquid phase medium into the corresponding alkanoate and excretes this compound into the aqueous phase. The rate of octanoic acid production by the recombinant E. coli is equal to or better than the alkane oxidation rate of P. oleovorans, suggesting that two-liquid phase fermentations with E. coli might have future industrial applications.

引用

页码：367 / 371

页数：5

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