RELATIONSHIP OF COLONIC LUMINAL SHORT-CHAIN FATTY-ACIDS AND PH TO IN-VIVO CELL-PROLIFERATION IN RATS

The mechanism by which fermentable fibers may stimulate colonic cell proliferation was tested using two types of fiber (highly fermentable pectin, and less fermentable wheat bran), measuring in vivo concentrations of short-chain fatty acids (SCFA) in the cecum and the proximal and distal colon of rats and correlating these concentrations with cell proliferation indices at the same locations within the intestine. Pectin supplementation resulted in higher concentrations of propionate in proximal and distal colon as compared with fiber-free controls, whereas wheat bran resulted in a higher concentration of butyrate at every site. In the cecum, pH had the strongest correlation to indices of cell proliferation. The lower the pH the greater the number of cells per crypt column (P < 0.05), cells per crypt circumference (P < 0.01), and total number of cells per crypt (P < 0.001). Butyrate had the strongest correlation between a specific SCFA and indices of cell proliferation. In the distal colon, butyrate concentration was positively correlated with number of cells per crypt column (P < 0.05) and total number of cells per crypt (P < 0.05). This study shows that different fibers are fermented to different SCFA in different amounts and that the in vivo concentration of certain of these SCFA is significantly correlated with cell proliferation indices.