THE DEVELOPMENT OF A NEW METHODOLOGY FOR THE ASSESSMENT OF SPECIFIC METHANOGENIC ACTIVITY

被引：61

作者：

JAMES, A

CHERNICHARO, CAL

CAMPOS, CMM

机构：

[1] Department of Civil Engineering, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU England, Claremont Road

来源：

WATER RESEARCH | 1990年 / 24卷 / 07期

关键词：

anaerobic sludge; methane production; methodology; specific methanogenic activity; Warburg respirometer;

D O I：

10.1016/0043-1354(90)90131-O

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The measurement of the potential of anaerobic sludges to convert soluble substrate into CH4 and CO2 has mainly been carried out in two different ways: firstly, by the so called "specific methanogenic activity test" (SMA test), through the methane production rate and, secondly, by the assessment of the coenzyme F420 contents in the sludge. However, a very poor correlation was found between the measurements of F420 concentration and the SMA test carried out using acetate as substrate, Dolfing and Mulder (Appl. envir. Microbiol. 49, 1142-1145, 1985). Therefore, improvements of the SMA test through a methodology that could easily be controlled and standardized, would certainly lead to more reliable results. This paper presents a new methodology for the SMA test, which fits the major requirements necessary for an accurate performance of the test. Several experiments utilizing different sludges were carried out, and the results were very consistent leading to the conclusion that this method can be applied to any type of anaerobic sludge and can be easily standardized. © 1990.

引用

页码：813 / 825

页数：13

共 18 条

[11]

GHOSH S, 1987, STABILIZATION SEWAGE, P1

[12] RELATIONSHIP BETWEEN METHANOGENIC COFACTOR CONTENT AND MAXIMUM SPECIFIC METHANOGENIC ACTIVITY OF ANAEROBIC GRANULAR SLUDGES [J].

GORRIS, LG ;

DEKOK, TM ;

KROON, BM ;

VANDERDRIFT, C ;

VOGELS, GD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (05) :1126-1130

[13]

MCCARTY PL, 1963, J WATER POLLUT CON F, V35, P1501

[14] LIGHT AND ELECTRON-MICROSCOPIC EXAMINATIONS OF METHANE-PRODUCING BIOFILMS FROM ANAEROBIC FIXED-BED REACTORS [J].

ROBINSON, RW ;

AKIN, DE ;

NORDSTEDT, RA ;

THOMAS, MV ;

ALDRICH, HC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (01) :127-136

[15]

Umbreit WW., 1964, MANOMETRIC TECHNIQUE

[16]

VALCKE D, 1983, J WATER POLLUT CON F, V55, P1191

[17]

WEAST RC, 1980, HDB CHEM PHYSICS

[18] KINETIC-STUDY AND MATHEMATICAL-MODELING OF METHANOGENESIS OF ACETATE USING PURE CULTURES OF METHANOGENS [J].

YANG, ST ;

OKOS, MR .

BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (05) :661-667

← 1 2 →