CLONING AND CHARACTERIZATION OF A GENE FROM BACILLUS-STEAROTHERMOPHILUS VAR NON-DIASTATICUS ENCODING A GLYCEROL DEHYDROGENASE

被引：22

作者：

MALLINDER, PR ^{[1
]}

PRITCHARD, A ^{[1
]}

MOIR, A ^{[1
]}

机构：

[1] UNIV SHEFFIELD,KREBS INST,DEPT MOLEC BIOL & BIOTECHNOL,POB 594,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND

来源：

GENE | 1992年 / 110卷 / 01期

关键词：

RECOMBINANT DNA; THERMOPHILE; KINASE; ZYMOMONAS; CLOSTRIDIUM;

D O I：

10.1016/0378-1119(92)90438-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A 4.1-kb EcoRI fragment which includes the gene (gldA) encoding a glycerol dehydrogenase (GlDH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from Bacillus stearothermophilus var. non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to Escherichia coli glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD) mutants. Sequencing suggests that the gldA gene is likely to be monocistronic and encodes a protein of 39450 Da. The deduced amino acid composition and sequence of GlDH reveals that the protein is extremely similar to a characterized metal-dependent NAD-dependent GlDH from B. stearothermophilus RS93. The enzyme has limited homology to the iron-activated alcohol dehydrogenase of Zymomonas mobilis and the butanol dehydrogenase of Clostridium acetobutylicum.

引用

页码：9 / 16

页数：8

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