BIOCHEMICAL-CHARACTERIZATION OF THE METALLO-BETA-LACTAMASE CCRA FROM BACTEROIDES-FRAGILIS TAL3636

被引：87

作者：

YANG, YJ ^{[1
]}

RASMUSSEN, BA ^{[1
]}

BUSH, K ^{[1
]}

机构：

[1] AMER CYANAMID CORP,DIV MED RES,PEARL RIVER,NY 10965

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1992年 / 36卷 / 05期

关键词：

D O I：

10.1128/AAC.36.5.1155

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The CcrA beta-lactamase from Bacteroides fragilis TAL3636 was cloned into Escherichia coli and purified from inclusion bodies. This group 3 metalloenzyme hydrolyzed most beta-lactam antibiotics, including cephamycins and carbapenems. Following inhibition by chelators, enzyme activity was recovered with the cations Zn2+ and Co2+. Clavulanate and sulbactam were activators; tazobactam at 10-mu-M inactivated the enzyme.

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页码：1155 / 1157

页数：3

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