A 2-SITE MONOCLONAL-ANTIBODY ELISA FOR THE QUANTIFICATION OF GROUP-V ALLERGENS IN GRASS EXTRACTS

被引：25

作者：

FAHLBUSCH, B ^{[1
]}

SCHLENVOIGT, G ^{[1
]}

MULLER, WD ^{[1
]}

WEBER, B ^{[1
]}

JAGER, L ^{[1
]}

机构：

[1] ALLERGOPHARMA JOACHIM GANZER KG, REINBEK, GERMANY

来源：

CLINICAL AND EXPERIMENTAL ALLERGY | 1994年 / 24卷 / 08期

关键词：

D O I：

10.1111/j.1365-2222.1994.tb00986.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

A two-site solid-phase enzymimmunoassay was used for the quantification of group V allergen in grass pollen extracts in mass units. The assay is based on the monoclonal antibodies (MoAbs), 1D11 and 3B2 which recognize different epitopes on the standard Phl p V. The MoAb-ELISA is very sensitive (15-100 ng/ml Phl p V) and highly specific for group V allergens. Six pollen extracts of different grasses demonstrated parallel binding curves. The group V content ranged between 73 and 673 mu g/ml in the extracts. The International Standard of Phleum pratense (IS 82/520) contains 400 mu g/ml Phl p V. A good correlation was observed between the group V content and RAST inhibition, with the exception of Poa pratensis.

引用

页码：752 / 757

页数：6

共 21 条

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