Identification of the nicking tyrosine of geminivirus Rep protein

被引：66

作者：

Laufs, J

Schumacher, S

Geisler, N

Jupin, I

Gronenborn, B

机构：

[1] CNRS, INST SCI VEGETALES, F-91198 GIF SUR YVETTE, FRANCE

[2] NIDDK, PHYS CHEM LAB, BETHESDA, MD 20892 USA

[3] MAX PLANCK INST BIOPHYS CHEM, D-37077 GOTTINGEN, GERMANY

来源：

FEBS LETTERS | 1995年 / 377卷 / 02期

关键词：

plant DNA virus; replication initiation; nucleotidyl transferases; covalent protein DNA link; rolling circle replication;

D O I：

10.1016/0014-5793(95)01355-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The replication initiator (Rep) proteins of geminiviruses perform a DNA cleavage and strand transfer reaction at the viral origin of replication, As a reaction intermediate, Rep proteins become covalently linked to the 5' end of the cleaved DNA, We have used tomato yellow leaf curl virus Rep protein for in vivo and in vitro analyses, Isolating a covalent peptide-nucleotide complex, we have identified the amino acid of Rep which mediates cleavage and links the protein to DNA, We show that tyrosine-103, located in a conserved sequence motif, initiates DNA cleavage and is the physical link between geminivirus Rep protein and its origin DNA.

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页码：258 / 262

页数：5

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