QUANTITATION OF MU-OPIOID RECEPTOR (MOR-1) MESSENGER-RNA IN SELECTED REGIONS OF THE RAT CNS

被引：23

作者：

BRODSKY, M ^{[1
]}

ELLIOTT, K ^{[1
]}

HYNANSKY, A ^{[1
]}

JENAB, S ^{[1
]}

INTURRISI, CE ^{[1
]}

机构：

[1] MEM SLOAN KETTERING CANC CTR, DEPT NEUROL, PAIN SERV, NEW YORK, NY 10021 USA

来源：

NEUROREPORT | 1995年 / 6卷 / 05期

关键词：

MU OPIOID RECEPTOR MESSENGER-RNA; ADULT RAT; BRAIN REGIONS; SPINAL CORD; SOLUTION HYBRIDIZATION; RNA QUANTITATION;

D O I：

10.1097/00001756-199503270-00005

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE mu opioid receptor (MOR-1) mRNA was quantified in rat CNS by a sensitive solution hybridization (SH) technique, employing a P-32-labeled riboprobe derived from the coding region of MOR-1 cDNA. In a Northern blot analysis this riboprobe hybridized to a 14 kb form of rat MOR-1 mRNA. The linear range of SH assay extends from 1 to 250 pg of MOR-1 sense transcript (equivalent to 9.3-2325 pg of MOR-1 mRNA). A microdissection technique for reproducible sampling of selected CNS regions, followed by the SH assay, allowed for a quantitative study of MOR-1 mRNA distribution. The highest levels of MOR-1 mRNA were present in medial thalamus (17.8 +/- 0.3 pg/mu g RNA), and the lowest in the cerebellum (0.4 +/- 0.1 pg/mu g RNA). Hypothalamus, dorsal spinal horn, nucleus raphe, periaqueductal gray, and sensorimotor cortex contained intermediate levels. This distribution closely parallels the pattern of mu receptor binding, suggesting that both the mRNA and the receptor protein are colocalized within most of the regions studied.

引用

页码：725 / 729

页数：5

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