GENE-EXPRESSION OF TYPE-I PHOSPHOLIPASE-A2 IN PANCREATIC BETA-CELLS - REGULATION OF MESSENGER-RNA LEVELS BY STARVATION OR GLUCOSE EXCESS

被引：31

作者：

METZ, S

HOLMES, D

ROBERTSON, RP

LEITNER, W

DRAZNIN, B

机构：

[1] VET ADM MED CTR,DENVER,CO 80220

[2] UNIV MINNESOTA,MINNEAPOLIS,MN 55455

[3] WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT MED,MADISON,WI

[4] WILLIAM S MIDDLETON MEM VET ADM MED CTR,DIV ENDOCRINOL,MADISON,WI

[5] UNIV WISCONSIN,DEPT MED,MADISON,WI 53706

[6] UNIV WISCONSIN,DIV ENDOCRINOL,MADISON,WI 53706

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

关键词：

INSULIN; PANCREATIC ISLET; PHOSPHOLIPASE; GLUCOSE; BETA-CELL; FASTING;

D O I：

10.1016/0014-5793(91)81397-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats; they decreased in islets cultured in a high glucose concentration (control values at 5.5 mM glucose = 150 +/- 6% of those at 22 mM) which impaired subsequent insulin secretion (reduction in second-phase release = 70 +/- 11%). These studies uniquely demonstrate that type I phospholipase A2 is expressed specifically in beta cells and that nutrient availability modulates transcript levels, an effect which could contribute to the detrimental influence of prolonged hyperglycemia on islet function.

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收藏

页码：110 / 112

页数：3

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