GENE-EXPRESSION OF TYPE-I PHOSPHOLIPASE-A2 IN PANCREATIC BETA-CELLS - REGULATION OF MESSENGER-RNA LEVELS BY STARVATION OR GLUCOSE EXCESS

被引：31

作者：

METZ, S

HOLMES, D

ROBERTSON, RP

LEITNER, W

DRAZNIN, B

机构：

[1] VET ADM MED CTR,DENVER,CO 80220

[2] UNIV MINNESOTA,MINNEAPOLIS,MN 55455

[3] WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT MED,MADISON,WI

[4] WILLIAM S MIDDLETON MEM VET ADM MED CTR,DIV ENDOCRINOL,MADISON,WI

[5] UNIV WISCONSIN,DEPT MED,MADISON,WI 53706

[6] UNIV WISCONSIN,DIV ENDOCRINOL,MADISON,WI 53706

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

关键词：

INSULIN; PANCREATIC ISLET; PHOSPHOLIPASE; GLUCOSE; BETA-CELL; FASTING;

D O I：

10.1016/0014-5793(91)81397-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats; they decreased in islets cultured in a high glucose concentration (control values at 5.5 mM glucose = 150 +/- 6% of those at 22 mM) which impaired subsequent insulin secretion (reduction in second-phase release = 70 +/- 11%). These studies uniquely demonstrate that type I phospholipase A2 is expressed specifically in beta cells and that nutrient availability modulates transcript levels, an effect which could contribute to the detrimental influence of prolonged hyperglycemia on islet function.

引用

页码：110 / 112

页数：3

共 26 条

[11] THE PRIMARY STRUCTURE OF A MEMBRANE-ASSOCIATED PHOSPHOLIPASE-A2 FROM HUMAN SPLEEN [J].