Surface reactive polymers for special applications in nucleic acid synthesis

被引：9

作者：

Seliger, H ^{[1
]}

Bader, R ^{[1
]}

BirchHirschfeld, E ^{[1
]}

FoldesPapp, Z ^{[1
]}

Guhrs, KH ^{[1
]}

Hinz, M ^{[1
]}

Rosch, R ^{[1
]}

Scharpf, C ^{[1
]}

机构：

[1] INST MOLEK BIOTECHNOL EV,D-07745 JENA,GERMANY

来源：

REACTIVE & FUNCTIONAL POLYMERS | 1995年 / 26卷 / 1-3期

关键词：

surface-reactive polymers; nucleic acid synthesis;

D O I：

10.1016/1381-5148(95)00059-O

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This publication reviews work done in the authors' laboratories on the functionalization of surface-reactive polymers and their application as a basis for the preparation of oligonucleotides of extended length, larger number or quantity. In addition to nonporous particulate materials (silicagel microbeads; polystyrene-grafted polytetrafluoroethylene particles) special focus is on recent work describing the introduction of spacered amino groups into polypropylene films and the preparation of oligonucleotides on polypropylene surface. A prototype apparatus is shown, for use with a four-column DNA synthesizer, that permits the semi-mechanized preparation of a one-dimensional array of oligonucleotides of variant sequence.

引用

页码：119 / 126

页数：8

共 16 条

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