THE EFFECT OF AGE ON BINDING OF MK-801 IN THE CAT VISUAL-CORTEX

被引:41
作者
GORDON, B
DAW, N
PARKINSON, D
机构
[1] WASHINGTON UNIV,SCH MED,DEPT ANAT & NEUROBIOL,ST LOUIS,MO 63110
[2] WASHINGTON UNIV,SCH MED,DEPT CELL BIOL,ST LOUIS,MO 63110
来源
DEVELOPMENTAL BRAIN RESEARCH | 1991年 / 62卷 / 01期
关键词
MK-801; VISUAL CORTEX; N-METHYL-D-ASPARTATE; MAGNESIUM BLOCKADE; AREA-17;
D O I
10.1016/0165-3806(91)90190-T
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have examined the effect of age on the binding of (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5,10-imine maleate (MK-801) in the cat visual cortex. We hypothesized that this binding might change with age because: (1) MK-801 binds to a site associated with the N-methyl-D-aspartate (NMDA) receptor; (2) the NMDA receptor complex has been implicated in neural plasticity; (3) plasticity in the cat visual cortex is age dependent. We used standard receptor binding techniques to measure MK-801 binding in membrane homogenates in cats aged 7 days (d), 21 d, 43 d, 83 d, 7-8 months (mo) and over 2 years. Glutamate (100-mu-M), glycine (30-mu-M) and spermidine (20-mu-M) were used to enhance binding. We found that MK-801 binding is maximal at about 6 weeks of age, decreases slightly by 83 days and then decreases more dramatically in adults. Saturation analysis showed that the variation of binding with age resulted from variation in number of binding sites and not from variation in affinity. The ability of Mg2+ to inhibit MK-801 binding did not change with age. Dark rearing did not alter the development of MK-801 binding sites.
引用
收藏
页码:61 / 67
页数:7
相关论文
共 32 条
[1]   LONG-TERM POTENTIATION AND NMDA RECEPTORS IN RAT VISUAL-CORTEX [J].
ARTOLA, A ;
SINGER, W .
NATURE, 1987, 330 (6149) :649-652
[2]  
BEAR MF, 1990, J NEUROSCI, V10, P909
[3]   THE DEVELOPMENT OF N-METHYL-D-ASPARTATE RECEPTORS IN CAT VISUAL-CORTEX [J].
BODEGREUEL, KM ;
SINGER, W .
DEVELOPMENTAL BRAIN RESEARCH, 1989, 46 (02) :197-204
[4]   BIOCHEMICAL-EVIDENCE THAT GLYCINE ALLOSTERICALLY REGULATES AN NMDA RECEPTOR-COUPLED ION CHANNEL [J].
BONHAUS, DW ;
BURGE, BC ;
MCNAMARA, JO .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1987, 142 (03) :489-490
[5]  
BOWERY NG, 1986, BRIT J PHARMACOL, P775
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   NMDA RECEPTORS - THEIR ROLE IN LONG-TERM POTENTIATION [J].
COLLINGRIDGE, GL ;
BLISS, TVP .
TRENDS IN NEUROSCIENCES, 1987, 10 (07) :288-293
[8]   POSTNATAL-DEVELOPMENT OF THE EXCITATORY AMINO-ACID SYSTEM IN VISUAL-CORTEX OF THE RAT - CHANGES IN LIGAND-BINDING TO NMDA, QUISQUALATE AND KAINATE RECEPTORS [J].
ERDO, SL ;
WOLFF, JR .
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 1990, 8 (02) :199-204
[9]   THE NOVEL ANTICONVULSANT MK-801 BINDS TO THE ACTIVATED STATE OF THE N-METHYL-D-ASPARTATE RECEPTOR IN RAT-BRAIN [J].
FOSTER, AC ;
WONG, EHF .
BRITISH JOURNAL OF PHARMACOLOGY, 1987, 91 (02) :403-409
[10]  
FOX K, 1989, J NEUROSCI, V9, P2443