HIGHLY POLYMORPHIC TETRAMER REPEAT (GATA)(N) ON HUMAN CHROMOSOME-11P15.3

被引：3

作者：

GEN, MW ^{[1
]}

PARRY, PJ ^{[1
]}

NING, Y ^{[1
]}

EVANS, GA ^{[1
]}

机构：

[1] SALK INST BIOL STUDIES,MOLEC GENET LAB,LA JOLLA,CA 92037

来源：

GENOMICS | 1993年 / 17卷 / 03期

关键词：

D O I：

10.1006/geno.1993.1405

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A tetrameric repeat sequence (GATA)(n) was identified by random DNA sequencing of chromosome 11-specific cosmid clones and located at 11p15.3 by fluorescence in situ hybridization. Oligonucleotide primers flanking the repetitive unit were used to amplify the DNA using the polymerase chain reaction (PCR) and this repetitive element was shown to be highly polymorphic and inherited in typical Mendelian fashion. Analysis of amplification products containing the repetitive element from 45 unrelated Caucasian individuals and five families showed at least five alleles at this locus, ranging from 227 to 249 bases in length. This polymorphism may serve as a useful PCR-detectable genetic marker for 11p15.3, a landmark for disease gene isolation, and a locus for identity testing. © 1993 Academic Press, Inc.

引用

页码：770 / 772

页数：3

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