CHARACTERIZATION OF THE ACUTE-PHASE SERUM-PROTEIN RESPONSE IN PIGS

被引：126

作者：

LAMPREAVE, F ^{[1
]}

GONZALEZRAMON, N ^{[1
]}

MARTINEZAYENSA, S ^{[1
]}

HERNANDEZ, MA ^{[1
]}

LORENZO, HK ^{[1
]}

GARCIAGIL, A ^{[1
]}

PINEIRO, A ^{[1
]}

机构：

[1] UNIV ZARAGOZA, FAC MED, DEPT CIRUG, ZARAGOZA, SPAIN

来源：

ELECTROPHORESIS | 1994年 / 15卷 / 05期

关键词：

Body fluids - Electrophoresis - Mammals - Pathology - Proteins - Sodium dodecyl sulfate;

D O I：

10.1002/elps.1150150195

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Acute inflammation was induced in pigs using a single subcutaneous turpentine injection. The acute phase serum protein response was analyzed using crossed immunoelectrophoresis and immunodiffusion. The concentration of C reactive protein and haptoglobin increases 5-7 times 48h after the injection, whereas the concentration of an alpha(2)-globulin, named pig major acute phase protein (pig-MAP), increases at least 15-fold. A molecular mass of 115 kDa for pig-MAP was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This protein did not crossreact with antisera to human hemopexin, ceruloplasmin, H-kininogen and complement factor C3. Albumin and alpha-lipoprotein were negative acute phase proteins because their concentration significantly decreased during inflammation. Finally, the concentration of alpha(1)-acid glycoprotein, fetuin, alpha(1)-protease inhibitor, transferrin and alpha(2)-macroglobulins, as well as total proteins, did not change significantly during inflammation.

引用

页码：672 / 676

页数：5

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