PARAMETERS INFLUENCING STABLE TRANSFORMATION OF RICE IMMATURE EMBRYOS AND RECOVERY OF TRANSGENIC PLANTS USING ELECTRIC-DISCHARGE PARTICLE-ACCELERATION

被引:19
作者
CHRISTOU, P [1 ]
FORD, T [1 ]
机构
[1] AGRACETUS,MIDDLETON,WI 53562
关键词
ORYZA SATIVA L; TRANSFORMATION PARAMETERS; TRANSGENIC INDICA AND JAPONICA PLANTS; PARTICLE BOMBARDMENT; BETA-GLUCURONIDASE;
D O I
10.1006/anbo.1995.1038
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Recovery of transgenic rice plants from elite varieties was reported from the authors' laboratory. By using electric discharge particle acceleration, recombinant technology was extended to commercially important japonica and indica rice cultivars not amenable to conventional transformation methodologies. Critical parameters influencing the recovery of transformed embryogenic callus from which transgenic plants were recovered have been defined. Such parameters included the physiological condition of explants prior to bombardment, DNA and gold particle loading rates, accelerating voltage, and depth of particle penetration. Selection for recovery of stable transformants was not critical in this transformation/regeneration system; both selected and non-selected tissues yielded transformed embryogenic callus and plants at approximately similar frequencies.
引用
收藏
页码:407 / 413
页数:7
相关论文
共 23 条
[1]   STABLE TRANSFORMATION OF SOYBEAN CALLUS BY DNA-COATED GOLD PARTICLES [J].
CHRISTOU, P ;
MCCABE, DE ;
SWAIN, WF .
PLANT PHYSIOLOGY, 1988, 87 (03) :671-674
[2]   PREDICTION OF GERM-LINE TRANSFORMATION EVENTS IN CHIMERIC R(O) TRANSGENIC SOYBEAN PLANTLETS USING TISSUE-SPECIFIC EXPRESSION PATTERNS [J].
CHRISTOU, P ;
MCCABE, DE .
PLANT JOURNAL, 1992, 2 (03) :283-290
[3]  
CHRISTOU P, 1993, IN VITRO CELL DEV-PL, V29P, P119
[4]   THE IMPACT OF SELECTION PARAMETERS ON THE PHENOTYPE AND GENOTYPE OF TRANSGENIC RICE CALLUS AND PLANTS [J].
CHRISTOU, P ;
FORD, TL .
TRANSGENIC RESEARCH, 1995, 4 (01) :44-51
[5]   PRODUCTION OF TRANSGENIC RICE (ORYZA-SATIVA L) PLANTS FROM AGRONOMICALLY IMPORTANT INDICA AND JAPONICA VARIETIES VIA ELECTRIC-DISCHARGE PARTICLE-ACCELERATION OF EXOGENOUS DNA INTO IMMATURE ZYGOTIC EMBRYOS [J].
CHRISTOU, P ;
FORD, TL ;
KOFRON, M .
BIO-TECHNOLOGY, 1991, 9 (10) :957-962
[6]   INHERITANCE AND EXPRESSION OF FOREIGN GENES IN TRANSGENIC SOYBEAN PLANTS [J].
CHRISTOU, P ;
SWAIN, WF ;
YANG, NS ;
MCCABE, DE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (19) :7500-7504
[8]  
CHRISTOU P, 1991, TRENDS BIOTECHNOL, V10, P239
[9]   GENETICALLY ENGINEERED FERTILE INDICA-RICE RECOVERED FROM PROTOPLASTS [J].
DATTA, SK ;
PETERHANS, A ;
DATTA, K ;
POTRYKUS, I .
BIO-TECHNOLOGY, 1990, 8 (08) :736-740
[10]   EMBRYOGENESIS AND PLANT-REGENERATION FROM MICROSPORES OF BOTH INDICA AND JAPONICA RICE (ORYZA-SATIVA) [J].
DATTA, SK ;
DATTA, K ;
POTRYKUS, I .
PLANT SCIENCE, 1990, 67 (01) :83-88