ESTABLISHING PARASEGMENTS IN DROSOPHILA EMBRYOS - ROLES OF THE ODD-SKIPPED AND NAKED GENES

被引:52
作者
MULLEN, JR [1 ]
DINARDO, S [1 ]
机构
[1] ROCKEFELLER UNIV, NEW YORK, NY 10021 USA
关键词
D O I
10.1006/dbio.1995.1145
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
During Drosophila embryogenesis, a genetic cascade establishes repeating developmental units, the parasegments, along the anterior-posterior axis. Anterior and posterior boundaries of parasegments are defined by narrow stripes of cells expressing the segment polarity genes engrailed and wingless, respectively. Through single and double mutant analysis, we describe genetic interactions regulating the precise activation of engrailed and wingless in alternate parasegments. The pair-rule gene odd-skipped and the segment polarity gene naked are both required to restrict engrailed expression. odd-skipped represses expression of fushi tarazu, a known activator of engrailed. naked prevents activation of engrailed by fushi tarazu, without affecting fushi tarazu expression. engrailed expression is thus limited to narrow stripes of cells at the anterior boundaries of these parasegments. wingless expression is regulated by both odd-skipped and the pair-rule gene paired. odd-skipped represses wingless expression, while paired restricts the domain of expression of odd-skipped, wingless expression is thus allowed in narrow stripes of cells at the posterior boundaries of these parasegments. Accurate expression of engrailed and wingless is also required for cells within each parasegment to assume their proper positional identity. In odd-skipped mutants, the positional identities of particular cells are changed, creating mirror-image duplications of the body pattern. We present a model describing how the altered expression patterns of fushi tarazu, engrailed, and wingless generate the mutant phenotype. (C) 1995 Academic Press, Inc.
引用
收藏
页码:295 / 308
页数:14
相关论文
共 51 条
[41]   A PROTEIN WITH SEVERAL POSSIBLE MEMBRANE-SPANNING DOMAINS ENCODED BY THE DROSOPHILA SEGMENT POLARITY GENE PATCHED [J].
NAKANO, Y ;
GUERRERO, I ;
HIDALGO, A ;
TAYLOR, A ;
WHITTLE, JRS ;
INGHAM, PW .
NATURE, 1989, 341 (6242) :508-513
[42]  
NOORDERMEER J, 1992, DEVELOPMENT, V116, P711
[43]   MUTATIONS AFFECTING SEGMENT NUMBER AND POLARITY IN DROSOPHILA [J].
NUSSLEINVOLHARD, C ;
WIESCHAUS, E .
NATURE, 1980, 287 (5785) :795-801
[44]   THE ENGRAILED LOCUS OF DROSOPHILA - STRUCTURAL-ANALYSIS OF AN EMBRYONIC TRANSCRIPT [J].
POOLE, SJ ;
KAUVAR, LM ;
DREES, B ;
KORNBERG, T .
CELL, 1985, 40 (01) :37-43
[45]  
POOLE SJ, 1988, DEVELOPMENT, V104, P85
[46]   EXPRESSION OF THE PROTOONCOGENE INT-1 IS RESTRICTED TO POSTMEIOTIC MALE GERM-CELLS AND THE NEURAL-TUBE OF MIDGESTATIONAL EMBRYOS [J].
SHACKLEFORD, GM ;
VARMUS, HE .
CELL, 1987, 50 (01) :89-95
[47]   CELL LINEAGE AND DEVELOPMENT IN THE LARVAL EPIDERMIS OF DROSOPHILA-MELANOGASTER [J].
SZABAD, J ;
SCHUPBACH, T ;
WIESCHAUS, E .
DEVELOPMENTAL BIOLOGY, 1979, 73 (02) :256-271
[48]  
WARRIOR R, 1990, DEVELOPMENT, V110, P759
[49]   SPATIAL REGULATION OF ENGRAILED EXPRESSION IN THE DROSOPHILA EMBRYO [J].
WEIR, MP ;
EDGAR, BA ;
KORNBERG, T ;
SCHUBIGER, G .
GENES & DEVELOPMENT, 1988, 2 (09) :1194-1203
[50]   EXPRESSION OF THE PROTOONCOGENE INT-1 IS RESTRICTED TO SPECIFIC NEURAL CELLS IN THE DEVELOPING MOUSE EMBRYO [J].
WILKINSON, DG ;
BAILES, JA ;
MCMAHON, AP .
CELL, 1987, 50 (01) :79-88