THE EFFECTS OF FURFURAL ON ETHANOL-PRODUCTION BY SACCHAROMYCES-CEREVISIAE IN BATCH CULTURE

被引:91
作者
BOYER, LJ [1 ]
VEGA, JL [1 ]
KLASSON, KT [1 ]
CLAUSEN, EC [1 ]
GADDY, JL [1 ]
机构
[1] UNIV ARKANSAS,DEPT CHEM ENGN,FAYETTEVILLE,AR 72701
关键词
ETHANOL; SACCHAROMYCES-CEREVISIAE; BATCH; FURFURAL; INHIBITION; MONOD KINETICS; INOCULUM SIZE;
D O I
10.1016/0961-9534(92)90018-L
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Browning reaction products such as furfural and 5-hydroxy-methyl-furfural (HMF) have been shown to inhibit microbial growth and metabolism in ethanol fermentations using Saccharomyces cerevisiae. This paper quantifies the extent of furfural inhibition on yeast growth, glucose utilization, and ethanol production as a function of inoculum size (0.1-9 gl-1). Batch culture experiments were conducted using furfural concentrations in the range of 0 to 2.0 gl-1 and mathematical correlations were proposed and tested. The results indicate that the specific growth rate decreased with increasing furfural concentration and inoculum size, while the maintenance coefficients were unaffected. The apparent and true cell yield coefficients on glucose were depressed with the addition of furfural. Specific production rates were unaffected at the furfural levels used but ethanol inhibition was apparent. The specific production rate was less inhibited by ethanol at higher inoculum sizes. Global specific productivities were not affected by the presence of furfural. At a 0.1 gl-1 inoculum size, furfural depletion was complete within 15-20 h, depending upon the furfural concentration employed. At higher inoculum levels (2-9 gl-1), all furfural was depleted in less than 5 h.
引用
收藏
页码:41 / 48
页数:8
相关论文
共 17 条
[1]  
Banerjee, Viswanathan, Effect of furfural and 5-hydroxy-methyl-furfural on the growth and alcohol production by yeast, Proceedings of the 40th Annual Convention of Sugar Technologists' Association of India, (1974)
[2]  
Azhar, Bery, Colcord, Roberts, Corbitt, Factors affecting alcohol fermentation of wood acid hydrolysate, Biotechnol. Bioeng. Symp., 11, pp. 293-300, (1981)
[3]  
Chung, Lee, Ethanol fermentation of crude acid hydrolyzate of cellulose using high-level yeast inocula, Biotechnol. Bioeng., 27, 3, pp. 308-315, (1985)
[4]  
Vitrinskaya, Soboleva, Influence of furfural on the energy metabolism of fodder yeasts, Appl. Biochem. Microbiol., 11, 5, pp. 579-582, (1975)
[5]  
Banerjee, Bhatnagar, Viswanathan, Inhibition of glycolysis by furfural in Saccharomyces cerevisiae, Eur. J. Appl. Microbiol. Biotechnol., 11, 4, pp. 226-228, (1981)
[6]  
Bazin, Lag phases and transentients, Microbial Population Dynamics, pp. 71-74, (1982)
[7]  
Monod, The growth of bacterial cultures, Annual Review of Microbiology, 3, pp. 371-394, (1949)
[8]  
Andrews, A mathematical model for the continuous culture on microorganisms utilizing inhibitory substrates, Biotechnology and Bioengineering, 10, 6, pp. 707-723, (1968)
[9]  
Vega, Navarro, Clausen, Gaddv, Effects of inoculum size on ethanol inhibition modeling and other fermentation parameters, Biotechnol. Bioeng., 29, 5, pp. 633-638, (1987)
[10]  
Aiba, Shoda, Nagatini, Kinetics of product inhibition in alcohol fermentation, Biotechnology and Bioengineering, 10, 6, pp. 845-864, (1968)