THE EFFECTS OF FURFURAL ON ETHANOL-PRODUCTION BY SACCHAROMYCES-CEREVISIAE IN BATCH CULTURE

被引:91
作者
BOYER, LJ [1 ]
VEGA, JL [1 ]
KLASSON, KT [1 ]
CLAUSEN, EC [1 ]
GADDY, JL [1 ]
机构
[1] UNIV ARKANSAS,DEPT CHEM ENGN,FAYETTEVILLE,AR 72701
关键词
ETHANOL; SACCHAROMYCES-CEREVISIAE; BATCH; FURFURAL; INHIBITION; MONOD KINETICS; INOCULUM SIZE;
D O I
10.1016/0961-9534(92)90018-L
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Browning reaction products such as furfural and 5-hydroxy-methyl-furfural (HMF) have been shown to inhibit microbial growth and metabolism in ethanol fermentations using Saccharomyces cerevisiae. This paper quantifies the extent of furfural inhibition on yeast growth, glucose utilization, and ethanol production as a function of inoculum size (0.1-9 gl-1). Batch culture experiments were conducted using furfural concentrations in the range of 0 to 2.0 gl-1 and mathematical correlations were proposed and tested. The results indicate that the specific growth rate decreased with increasing furfural concentration and inoculum size, while the maintenance coefficients were unaffected. The apparent and true cell yield coefficients on glucose were depressed with the addition of furfural. Specific production rates were unaffected at the furfural levels used but ethanol inhibition was apparent. The specific production rate was less inhibited by ethanol at higher inoculum sizes. Global specific productivities were not affected by the presence of furfural. At a 0.1 gl-1 inoculum size, furfural depletion was complete within 15-20 h, depending upon the furfural concentration employed. At higher inoculum levels (2-9 gl-1), all furfural was depleted in less than 5 h.
引用
收藏
页码:41 / 48
页数:8
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