ERROR ANALYSIS IN MANUAL AND AUTOMATED DNA SEQUENCING

被引：11

作者：

KHURSHID, F ^{[1
]}

BECK, S ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,44 LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 208卷 / 01期

关键词：

D O I：

10.1006/abio.1993.1019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The frequency, position, and type of errors were analyzed for sequence data which were generated with the help of (I) a manual digitizer, (II) an off-line automated film reader, and (III) an on-line automated gel reader. About 400 film/gel readings were analyzed and the results were used to generate error profiles for each method. The profiles identify several method- and project-specific problem areas and provide useful guidance for the assessment of DNA sequence data in general. © 1993 Academic Press, Inc.

引用

页码：138 / 143

页数：6

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