ATP-ACTIVATED CA2+-PERMEABLE CHANNELS IN RAT PERITONEAL-MACROPHAGES

被引：24

作者：

NAUMOV, AP ^{[1
]}

KURYSHEV, YA ^{[1
]}

KAZNACHEYEVA, EV ^{[1
]}

MOZHAYEVA, GN ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI, INST CYTOL, TIKHORETSKY AVE 4, 194064 ST PETERSBURG, USSR

来源：

FEBS LETTERS | 1992年 / 313卷 / 03期

关键词：

MACROPHAGE; ATP; PATCH CLAMP; CALCIUM CHANNEL;

D O I：

10.1016/0014-5793(92)81210-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The patch-clamp technique was used to study mechanisms of ATP-induced Ca2+ influx in rat peritoneal macrophages. The experiments on whole-cell and patch membranes have shown that extracellular ATP activates channels permeable to di- and monovalent inorganic cations, Ratios of unitary channel conductances in 105 mM Ca2+, Sr2+, Mn2+, Ba2+ and normal sodium solutions were 1.0, 0.95, 0.75, 0.55 and 0.85, respectively. The channels could open in the presence of non-hydrolyzable GTP analogues in artificial intracellular solution. The data are consistent with the hypothesis that a GTP-binding protein is involved in receptor-to-channel coupling.

引用

页码：285 / 287

页数：3

共 11 条

[1] Conrad R.E., 1981, MANUAL MACROPHAGES M
[2] FABIATO A, 1979, J PHYSIOL-PARIS, V75, P463
[3] GAMALEY IA, 1991, CYTOLOGY, V33, P69
[4] GILMAN AG, 1987, ANNU REV BIOCHEM, V56, P615, DOI 10.1146/annurev.bi.56.070187.003151
[5] GORDON S, 1988, J CELL SCI, P1
[6] GREENBERG S, 1988, J BIOL CHEM, V263, P10337
[7] VARIETY OF CA2+-PERMEABLE CHANNELS IN HUMAN CARCINOMA A431-CELLS
MOZHAYEVA, GN
NAUMOV, AP
KURYSHEV, YA
[J]. JOURNAL OF MEMBRANE BIOLOGY, 1991, 124 (02) : 113 - 126
[8] EXTRACELLULAR ATP ITSELF ELICITS SUPEROXIDE GENERATION IN GUINEA-PIG PERITONEAL-MACROPHAGES
NAKANISHI, M
TAKIHARA, H
MINORU, Y
YAGAWA, K
[J]. FEBS LETTERS, 1991, 282 (01) : 91 - 94
[9] BIPHASIC INCREASE IN INTRACELLULAR CALCIUM INDUCED BY PLATELET-ACTIVATING FACTOR IN MACROPHAGES
RANDRIAMAMPITA, C
TRAUTMANN, A
[J]. FEBS LETTERS, 1989, 249 (02) : 199 - 206
[10] STEINBERG TH, 1990, ANN NY ACAD SCI, V603, P120

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