PURIFICATION AND EXPANSION OF HUMAN SCHWANN-CELLS IN-VITRO

被引：130

作者：

RUTKOWSKI, JL ^{[1
]}

KIRK, CJ ^{[1
]}

LERNER, MA ^{[1
]}

TENNEKOON, GI ^{[1
]}

机构：

[1] CAMBRIDGE NEUROSCI INC,CAMBRIDGE,MA 02139

来源：

NATURE MEDICINE | 1995年 / 1卷 / 01期

关键词：

D O I：

10.1038/nm0195-80

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability to culture cells from the human nervous system provides new insight into the pathophysiology of neurological diseases and could be crucial to the development of gene replacement therapies and neural transplantation. We report that the proliferation of human Schwann cells isolated from paediatric and adult nerves is sustained in vitro by recombinant glial growth factor. Agents that increase intracellular cyclic cAMP were also mitogenic towards Schwann cells but suppress growth of contaminating fibroblasts. As the lifespan of highly enriched cultures can be extended for up to twelve population doublings, large numbers of cells can be generated from nerve biopsies.

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页码：80 / 83

页数：4

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