COLORIMETRIC DETERMINATION OF MACROLIDE ANTIBIOTICS USING FERRIC ION

被引：12

作者：

GALLAGHER, PA ^{[1
]}

DANIELSON, ND ^{[1
]}

机构：

[1] MIAMI UNIV, DEPT CHEM, OXFORD, OH 45056 USA

来源：

TALANTA | 1995年 / 42卷 / 10期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0039-9140(95)98348-J

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Macrolide antibiotics such as erythromycin, oleandomycin, spiromycin, and tylosin are found to react with Fe3+ in the presence of an acetic acid-sulfuric acid mixture to form a colored product having a useful absorption band at 592 nm. Troleandomycin forms only a weakly colored product upon reaction. The molar absorptivity is about 2900 l mol(-1) cm(-1) for erythromycin and the detection limit is 5 mu g ml(-1). This colorimetric method permits the analysis of fermentation broths containing either erythromycin or tylosin without a separation step.

引用

页码：1425 / 1432

页数：8

共 11 条

[1]

BURKE RW, 1974, CLIN CHEM, V20, P794

[2] A STUDY OF ION-PAIR FORMATION BETWEEN ERYTHROMYCIN AND BROMOTHYMOL BLUE, METHYLTHYMOL BLUE, AND THYMOL BLUE AND THEIR USE FOR ASSAYING ERYTHROMYCIN IN DOSAGE FORMS [J].