DYNAMICS OF HYDROGEN-ATOMS IN SUPEROXIDE-DISMUTASE BY QUASI-ELASTIC NEUTRON-SCATTERING

被引：64

作者：

ANDREANI, C

FILABOZZI, A

MENZINGER, F

DESIDERI, A

DERIU, A

DICOLA, D

机构：

[1] UNIV ROMA TOR VERGATA,DIPARTIMENTO FIS,I-00133 ROME,ITALY

[2] UNIV MESSINA,DIPARTIMENTO CHIM ORGAN & BIOL,I-98166 MESSINA,ITALY

[3] UNIV PARMA,DIPARTIMENTO FIS,I-43100 PARMA,ITALY

来源：

BIOPHYSICAL JOURNAL | 1995年 / 68卷 / 06期

关键词：

D O I：

10.1016/S0006-3495(95)80434-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The low energy dynamics of the enzyme Cu,Zn superoxide dismutase have been investigated by means of quasielastic neutron scattering in the temperature range 4-320 K. Below 200 K the scattering is purely elastic, while above this temperature a pronounced decrease in the elastic intensity is observed, together with the onset of a small quasielastic component. This behavior is similar to that previously observed in other more flexible globular proteins, and can be attributed to transitions between slightly different conformational substates of the protein tertiary structure. The presence of only a small quasielastic component, whose intensity is less than or equal to 25% of the total spectrum, is related to the high structural rigidity of this protein.

引用

页码：2519 / 2523

页数：5

共 24 条

[11] TEMPERATURE-DEPENDENT X-RAY-DIFFRACTION AS A PROBE OF PROTEIN STRUCTURAL DYNAMICS
FRAUENFELDER, H
PETSKO, GA
TSERNOGLOU, D
[J]. NATURE, 1979, 280 (5723) : 558 - 563
[12] PICOSECOND TIMESCALE RIGID-HELIX AND SIDE-CHAIN MOTIONS IN DEOXYMYOGLOBIN
FUROISCORBIN, S
SMITH, JC
KNELLER, GR
[J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1993, 16 (02): : 141 - 154
[13] PROTEIN AND PROTEIN-BOUND WATER DYNAMICS STUDIED BY RAYLEIGH-SCATTERING OF MOSSBAUER RADIATION (RSMR)
GOLDANSKII, VI
KRUPYANSKII, YF
[J]. QUARTERLY REVIEWS OF BIOPHYSICS, 1989, 22 (01) : 39 - 92
[14] OBSERVATION OF ACOUSTIC UMKLAPP PHONONS IN WATER-STABILIZED DNA BY NEUTRON-SCATTERING
GRIMM, H
STILLER, H
MAJKRZAK, CF
RUPPRECHT, A
DAHLBORG, U
[J]. PHYSICAL REVIEW LETTERS, 1987, 59 (15) : 1780 - 1783
[15] 3-DIMENSIONAL STRUCTURE OF CU,ZN-SUPEROXIDE DISMUTASE FROM SPINACH AT 2.0 A RESOLUTION
KITAGAWA, Y
TANAKA, N
HATA, Y
KUSUNOKI, M
LEE, GP
KATSUBE, Y
ASADA, K
AIBARA, S
MORITA, Y
[J]. JOURNAL OF BIOCHEMISTRY, 1991, 109 (03) : 477 - 485
[16] MCCORD JM, 1969, J BIOL CHEM, V224, P6049
[17] PARAK F, 1981, J MOL BIOL, V145, P824
[18] ATOMIC STRUCTURES OF WILD-TYPE AND THERMOSTABLE MUTANT RECOMBINANT HUMAN CU,ZN SUPEROXIDE-DISMUTASE
PARGE, HE
HALLEWELL, RA
TAINER, JA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (13) : 6109 - 6113
[19] DYNAMICS OF PROTEIN HYDRATION BY QUASI-ELASTIC NEUTRON-SCATTERING
RANDALL, J
MIDDENDORF, HD
CRESPI, HL
TAYLOR, AD
[J]. NATURE, 1978, 276 (5688) : 636 - 638
[20] MOLECULAR-SPECTROSCOPY AND BAYESIAN SPECTRAL-ANALYSIS - HOW MANY LINES ARE THERE
SIVIA, DS
CARLILE, CJ
[J]. JOURNAL OF CHEMICAL PHYSICS, 1992, 96 (01) : 170 - 178

← 1 2 3 →