DIFFERENCES BETWEEN THE ELECTRIC-FIELDS OF THE CATALYTIC SITES OF PAPAIN AND ACTINIDIN DETECTED BY USING THE THIOL-LOCATED NITROBENZOFURAZAN LABEL AS A SPECTROSCOPIC REPORTER GROUP

被引：16

作者：

BROCKLEHURST, K

SALIH, E

LODWIG, TS

机构：

来源：

BIOCHEMICAL JOURNAL | 1984年 / 220卷 / 02期

关键词：

D O I：

10.1042/bj2200609

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：609 / 612

页数：4

共 20 条

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[3] HIGHLY ELECTROPHILIC CHARACTER OF 4-CHLORO-7-NITROBENZOFURAZAN AND POSSIBLE CONSEQUENCES FOR ITS APPLICATIONS AS A PROTEIN-LABELING REAGENT
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[6] COOPERATIVE IONIZATION OF ASPARTIC-ACID-158 AND HISTIDINE-159 IN PAPAIN - EVIDENCE FROM F-19 NUCLEAR-MAGNETIC-RESONANCE AND FLUORESCENCE SPECTROSCOPY
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[7] REACTIVITY OF SH GROUPS WITH A FLUOROGENIC REAGENT
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[8] DIFFERENCES IN THE INTERACTIONS OF THE CATALYTIC GROUPS OF THE ACTIVE-CENTERS OF ACTINIDIN AND PAPAIN - RAPID PURIFICATION OF FULLY ACTIVE ACTINIDIN BY COVALENT CHROMATOGRAPHY AND CHARACTERIZATION OF ITS ACTIVE-CENTER BY USE OF 2-PROTONIC-STATE REACTIVITY PROBES
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MALTHOUSE, JPG
[J]. BIOCHEMICAL JOURNAL, 1981, 197 (03) : 739 - 746
[9] A MARKED GRADATION IN ACTIVE-CENTER PROPERTIES IN THE CYSTEINE PROTEINASES REVEALED BY NEUTRAL AND ANIONIC REACTIVITY PROBES - REACTIVITY CHARACTERISTICS OF THE THIOL-GROUPS OF ACTINIDIN, FICIN, PAPAIN AND PAPAYA PEPTIDASE-A TOWARDS 4,4'-DIPYRIDYL DISULFIDE AND 585'-DITHIOBIS-(2-NITROBENZOATE) DIANION
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[J]. BIOCHEMICAL JOURNAL, 1983, 209 (03) : 873 - 879
[10] Brocklehurst K., 1981, Topics in Enzyme and Fermentation Biotechnology, V5, P262

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